Recombination and positive selection identified in complete genome sequences of Japanese encephalitis virus

被引:0
作者
Jennifer Carney
Janet M. Daly
Ananda Nisalak
Tom Solomon
机构
[1] Institute of Infection and Global Health,Brain Infections Group, Department of Clinical Infection, Microbiology and Immunology
[2] University of Liverpool,School of Veterinary Medicine and Science
[3] Armed Forces Research Institute of Medical Sciences (AFRIMS),undefined
[4] University of Nottingham,undefined
来源
Archives of Virology | 2012年 / 157卷
关键词
West Nile Virus; Complete Genome Sequence; Dengue Virus; Japanese Encephalitis Virus; Human Isolate;
D O I
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中图分类号
学科分类号
摘要
The mosquito-borne Japanese encephalitis virus (JEV) causes encephalitis in man but not in pigs. Complete genomes of a human, mosquito and pig isolate from outbreaks in 1982 and 1985 in Thailand were sequenced with the aim of identifying determinants of virulence that may explain the differences in outcomes of JEV infection between pigs and man. Phylogenetic analysis revealed that five of these isolates belonged to genotype I, but the 1982 mosquito isolate belonged to genotype III. There was no evidence of recombination among the Thai isolates, but there were phylogenetic signals suggestive of recombination in a 1994 Korean isolate (K94P05). Two sites of the genome under positive selection were identified: codons 996 and 2296 (amino acids 175 of the non-structural protein NS1 and 24 of NS4B, respectively). A structurally significant substitution was seen at NS4B position 24 of the human isolate compared with the mosquito and pig isolates from the 1985 outbreak in Thailand. The potential importance of the two sites in the evolution and ecology of JEV merits further investigation.
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页码:75 / 83
页数:8
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