Immunogenicity of the capsid protein VP2 from porcine parvovirus expressed in low alkaloid transgenic tobacco

被引:0
|
作者
R.T. Rymerson
L. Babiuk
R. Menassa
B. Vanderbeld
J.E. Brandle
机构
[1] Agriculture and Agri-Food Canada,
[2] Veterinary and Infectious Disease Organization,undefined
来源
Molecular Breeding | 2003年 / 11卷
关键词
Low alkaloid; Porcine parvovirus; PPV; Transgenic tobacco; Vaccine;
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学科分类号
摘要
Porcine parvovirus is a widespread infectious viral disease with serious consequences to the reproductive health of swine. We have expressed the VP2 capsid protein of porcine parvovirus in the leaves of low alkaloid transgenic tobacco at approximately 0.3% of total soluble protein. Self-assembled virus-like particles were observed in planta by electron microscopy. Total soluble protein was extracted from the plant tissue and administered to mice by subcutaneous injection. An immune response was detected in these mice. The ability of serum antibodies to neutralize the infectivity of porcine parvovirus was further examined by a serum neutralization assay and was determined to be 1:2700–1:3900, a clear indication of the potential of VP2 expressed in plant material as a subunit vaccine against porcine parvovirus.
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页码:267 / 276
页数:9
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