Comparative transcriptome analysis of fertile and sterile buds from a genetically male sterile line of Chinese cabbage

被引:0
|
作者
Chang Liu
Zhiyong Liu
Chengyu Li
Yun Zhang
Hui Feng
机构
[1] Shenyang Agricultural University,College of Horticulture
来源
In Vitro Cellular & Developmental Biology - Plant | 2016年 / 52卷
关键词
Chinese cabbage; Male sterile; Transcriptome; DEGs; KEGG analysis;
D O I
暂无
中图分类号
学科分类号
摘要
In this study, we compared the transcriptome profiles of fertile versus sterile buds of a stably inherited genic male sterile AB line of Chinese cabbage using the Illumina high-throughput sequencing platform. Based on the analysis of gene expression levels, we detected 1013 differentially expressed genes (DEGs), including 907 that were upregulated and 106 that were downregulated in fertile buds. In addition, 481 genes were specifically differentially expressed. Among these, some DEGs were previously shown to play important roles in pollen and anther development, including AMS, MS2, ms35/myb26, and so on. We also performed gene ontology (GO) analysis to reveal the main biological functions of the DEGs. Only 19 GO terms were significantly enriched. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis was performed to uncover significantly enriched metabolic pathways and signal transduction pathways involving the DEGs, revealing five significantly enriched KEGG pathways that are pentose and glucuronate interconversions; alanine, aspartate, and glutamate metabolism; cysteine and methionine metabolism; ascorbate and aldarate metabolism; and starch and sucrose metabolism. These results could potentially explain the difference between fertile and sterile buds. Finally, we analyzed the expression patterns of 31 DEGs using qRT-PCR, which verified the reliability of the transcriptome sequencing results. Analysis of the transcriptome data provides valuable information for in-depth analysis of the molecular mechanism underlying pollen development, and it lays the foundation for clarifying the functions of these crucial genes and elucidating the sterility mechanism in Chinese cabbage.
引用
收藏
页码:130 / 139
页数:9
相关论文
共 50 条
  • [31] A comparative study on the anther development and microsporogenesis in male-fertile and male-sterile Chinese chive (Allium tuberosum)
    Li, CL
    Jiang, ZR
    Tong, XR
    Proceedings of the IVth International Symposium on Edible Alliaceae, 2005, (688): : 23 - 28
  • [32] Genome-wide analysis of the callose enzyme families of fertile and sterile flower buds of the Chinese cabbage (Brassica rapa L. ssp. pekinensis)
    Pu, Yanan
    Hou, Lingyun
    Guo, Yingqi
    Ullah, Ikram
    Yang, Yongping
    Yue, Yanling
    FEBS OPEN BIO, 2019, 9 (08): : 1432 - 1449
  • [33] Comparative transcript profiling of the fertile and sterile flower buds of pol CMS in B. napus
    An, Hong
    Yang, Zonghui
    Yi, Bin
    Wen, Jing
    Shen, Jinxiong
    Tu, Jinxing
    Ma, Chaozhi
    Fu, Tingdong
    BMC GENOMICS, 2014, 15
  • [34] Comparative transcript profiling of the fertile and sterile flower buds of pol CMS in B. napus
    Hong An
    Zonghui Yang
    Bin Yi
    Jing Wen
    Jinxiong Shen
    Jinxing Tu
    Chaozhi Ma
    Tingdong Fu
    BMC Genomics, 15
  • [35] Transcriptome Analysis of a Female-sterile Mutant (fsm) in Chinese Cabbage (Brassica campestris ssp pekinensis)
    Huang, Shengnan
    Liu, Zhiyong
    Li, Chengyu
    Yao, Runpeng
    Li, Danyang
    Hou, Li
    Li, Xiang
    Liu, Wenjie
    Feng, Hui
    FRONTIERS IN PLANT SCIENCE, 2017, 8
  • [36] Comparative analysis of morphology, cytology, and mitochondrial transcriptome of sterile male flowers of rubber tree
    Li, Fei
    Zhang, Yuhang
    Wang, Ying
    Gao, Heqiong
    Zhuang, Nansheng
    CROP SCIENCE, 2021, 61 (06) : 4055 - 4065
  • [37] COMPARATIVE EJACULATE INVESTIGATIONS ON MEN FROM STERILE AND FERTILE MARRIAGES
    STURDE, HC
    GLOWANIA, HJ
    BOHM, K
    ARCHIV FUR DERMATOLOGISCHE FORSCHUNG, 1971, 241 (04): : 426 - &
  • [38] ISOLATION AND ANALYSIS OF DIFFERENTIALLY EXPRESSED GENES BETWEEN MALE FERTILE AND MALE STERILE FLOWER BUDS OF MARIGOLD (TAGETES ERECTA L.)
    Hou, Zhiqiang
    Tang, Daocheng
    Tang, Nan
    PAKISTAN JOURNAL OF BOTANY, 2016, 48 (06) : 2423 - 2431
  • [39] Comparative transcriptome analysis of the cold resistance of the sterile rice line 33S
    Xie, Hongjun
    Zhu, Mingdong
    Yu, Yaying
    Zeng, Xiaoshan
    Tang, Guohua
    Duan, Yonghong
    Wang, Jianlong
    Yu, Yinghong
    PLOS ONE, 2022, 17 (01):
  • [40] De novo-based transcriptome profiling of male-sterile and fertile watermelon lines
    Rhee, Sun-Ju
    Kwon, Taehyung
    Seo, Minseok
    Jang, Yoon Jeong
    Sim, Tae Yong
    Cho, Seoae
    Han, Sang-Wook
    Lee, Gung Pyo
    PLOS ONE, 2017, 12 (11):