Induction of apoptosis by chemotherapeutic drugs: The role of FADD in activation of caspase-8 and synergy with death receptor ligands in ovarian carcinoma cells

被引：0

作者：

Milner A.E. ^{[1
]}

Palmer D.H. ^{[1
]}

Hodgkin E.A. ^{[1
]}

Eliopoulos A.G. ^{[1
]}

Knox P.G. ^{[1
]}

Poole C.J. ^{[1
]}

Kerr D.J. ^{[1
]}

Young L.S. ^{[1
]}

机构：

[1] CRC Institute for Cancer Studies, Clinical Research Block, University of Birmingham

来源：

Cell Death & Differentiation | 2002年 / 9卷 / 3期

基金：

英国医学研究理事会;

关键词：

Adenovirus; Apoptosis; Death receptors; Drug resistance; Ovarian carcinoma;

D O I：

10.1038/sj.cdd.4400945

中图分类号：

学科分类号：

摘要：

Although ovarian tumours initially respond to chemotherapy, they gradually acquire drug resistance. The aims of this study were to identify how chemotherapeutic drugs with diverse cellular targets activate apoptotic pathways and to investigate the mechanism by which exposure to a combination of drugs plus death receptor ligands can increase tumour cell kill. The results show that drugs with distinct cellular targets differentially up-regulate TRAIL and TNF as well CD95L, but do not require interaction of these ligands with their receptor partners to induce cell death. Factors that were critical in drug-induced apoptosis were activation of caspases, with caspase-8 being activated by diverse drugs in a FADD-independent manner. Certain drugs also demonstrated some dependence on FADD in the induction of cell death. Caspase-9 was activated more selectively by chemotherapeutic agents. Combining ligation of death receptors with exposure to drugs increased tumour cell kill in both drug resistant cell lines and primary ovarian carcinoma cells, even though these cells were not sensitive to death receptor ligation alone. CD95L was more consistent at combining with drugs than TRAIL or TNF. Investigation of the mechanism by which a combination of drugs plus CD95 ligation can increase cell death showed that caspase-8 was activated in cells exposed to a combination of cisplatin and anti-CD95, but not in cells exposed to either agent alone.

引用

页码：287 / 300

页数：13

共 44 条

[41]

Sun X.M., MacFarlane M., Zhuang J.G., Wolf B.B., Green D.R., Cohen G.M., Distinct caspase cascades are initiated in receptor-mediated and chemical-induced apoptosis, J. Biol. Chem., 274, pp. 5053-5060, (1999)

[42]

Engels I.H., Stepczynska A., Stroh C., Lauber K., Berg C., Schwenzer R., Wajant H., Janicke R.U., Porter A.G., Belka C., Gregor M., Schultze-Osthoff K., Wesselborg S., Caspase-8/FLICE functions as an executioner caspase in anticancer drug-induced apoptosis, Oncogene, 19, pp. 4563-4573, (2000)

[43]

Wieder T., Essmann F., Prokop A., Schmelz K., Schulze-Ostoff K., Beyaert R., Dorken B., Daniel P.T., Activation of caspase-8 in drug-induced apoptosis of B lymphoid cells is independent of CD95/Fas receptor-ligand interaction and occurs downstream of caspase-3, Blood, 97, pp. 1378-1387, (2001)

[44]

Griffiths G.J., Dubrez L., Morgan C.P., Jones N.A., Whitehouse J., Corfe B.M., Dive C., Hickman J., Cell damage-induced conformational changes of the pro-apoptotic protein Bak in vivo precede the onset of apoptosis, J. Cell. Biol., 144, pp. 903-914, (1999)

← 1 2 3 4 5 →