In Vitro Effects of Gamma Radiation from 60Co and 137Cs on Plasmid DNA

被引:0
作者
F. M. Milian
A. N. Gouveia
M. R. Gual
J. O. Echeimberg
J. D. T. Arruda-Neto
F. Garcia
A. C. G. Schenberg
E. J. Vicente
O. Rodriguez
F. Guzman
A. Deppman
机构
[1] Universidade de São Paulo,Instituto de Física
[2] Universidade de São Paulo,Instituto de Ciências Biomédicas
[3] Instituto Superior de Tecnologías y Ciencias Aplicadas (InSTEC),undefined
[4] Universidade Estadual de Santa Cruz,undefined
来源
Journal of Biological Physics | 2007年 / 33卷
关键词
Double-strand breaks; Single-strand breaks; Scavenger-derived radicals; Ionizing radiation; pBSKS + DNA; Gamma effects;
D O I
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中图分类号
学科分类号
摘要
The effects of gamma radiation from 60Co and 137Cs on DNA in aqueous solution are studied experimentally. Using an improved plasmid purification protocol and improved electrophoretic gel analysis techniques provided results with relatively small uncertainties. The results are compared with both theoretical and experimental results. In particular, the results obtained here are discussed in the light of recent discussion on supposed differences of the effects induced by gamma radiation from 60Co and 137Cs. We find that the effects of both types of gamma radiation are similar.
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页码:155 / 160
页数:5
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