Fluorescence lifetime-resolved imaging

被引：0
|
作者：
Yi-Chun Chen
Robert M. Clegg
机构：
[1] University of Illinois at Urbana-Champaign,Bioengineering Department
[2] University of Illinois at Urbana-Champaign,Center for Biophysics and Computational Biology
[3] University of Illinois at Urbana-Champaign,Department of Physics
[4] Loomis Laboratory of Physics,undefined
来源：
Photosynthesis Research | 2009年 / 102卷
关键词：
Fluorescence lifetime-resolved imaging; Microscopy; Model independent analysis; Fluorescence lifetime-resolved imaging microscopy; Fluorescence lifetime-resolved imaging; Spectral FLIM; Time-domain; Frequency domain;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
This is a short account of fluorescence lifetime-resolved imaging, in order to acquaint readers who are not experts with the basic methods for measuring lifetime-resolved signals throughout an image. We present the early FLI (fluorescence lifetime imaging) history, review shortly the instrumentation and experimental design, discuss briefly the fundamentals of the measured fluorescence response, and introduce the basic measurement methodologies. We also emphasize the complex nature of the fluorescence response in FLI signals, and introduce certain analysis methods that are appropriate and informative for complex fluorescence decays. The advantages of model independent analyses are discussed and examples given.
引用
收藏
页码：143 / 155
页数：12
相关论文
共 50 条
  • [31] Modulated electron-multiplied fluorescence lifetime imaging microscope: all-solid-state camera for fluorescence lifetime imaging
    Zhao, Qiaole
    Schelen, Ben
    Schouten, Raymond
    van den Oever, Rein
    Leenen, Rene
    van Kuijk, Harry
    Peters, Inge
    Polderdijk, Frank
    Bosiers, Jan
    Raspe, Marcel
    Jalink, Kees
    de Jong, Jan Geert Sander
    van Geest, Bert
    Stoop, Karel
    Young, Ian Ted
    JOURNAL OF BIOMEDICAL OPTICS, 2012, 17 (12)
  • [32] Assessing patterns for compressive fluorescence lifetime imaging
    Ochoa, M.
    Pian, O.
    Yao, R.
    Ducros, N.
    Intes, X.
    OPTICS LETTERS, 2018, 43 (18) : 4370 - 4373
  • [33] Fluorescence for spatially and temporally resolved electrochemical imaging
    Lewis, Todd H.
    Zhang, Bo
    CURRENT OPINION IN ELECTROCHEMISTRY, 2023, 40
  • [34] A comparative study of different instrumental concepts for spectrally and lifetime-resolved multiphoton intravital tomography (5D-IVT) in dermatological applications
    Schwarz, M.
    Riemann, I.
    Stracke, F.
    Huck, V.
    Gorzelanny, C.
    Schneider, S. W.
    Koenig, K.
    Puschmann, S.
    Lutz, V.
    Sommer, N.
    Rahn, C.
    Gallinat, S.
    Wenck, H.
    Wittern, K. -P.
    Fischer, F.
    IMAGING, MANIPULATION, AND ANALYSIS OF BIOMOLECULES, CELLS, AND TISSUES VIII, 2010, 7568
  • [35] Quantitative Analysis of Fluorescence Lifetime Measurements of the Macula Using the Fluorescence Lifetime Imaging Ophthalmoscope in Healthy Subjects
    Dysli, Chantal
    Quellec, Gwenole
    Abegg, Mathias
    Menke, Marcel N.
    Wolf-Schnurrbusch, Ute
    Kowal, Jens
    Blatz, Johannes
    La Schiazza, Olivier
    Leichtle, Alexander B.
    Wolf, Sebastian
    Zinkernagel, Martin S.
    INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE, 2014, 55 (04) : 2106 - 2113
  • [36] Maximizing Aggregation of Organic Fluorophores to Prolong Fluorescence Lifetime for Two-Photon Fluorescence Lifetime Imaging
    Hu, Wenbo
    Guo, Lihong
    Bai, Lei
    Miao, Xiaofei
    Ni, Yun
    Wang, Qi
    Zhao, Hui
    Xie, Meng
    Li, Lin
    Lu, Xiaomei
    Huang, Wei
    Fan, Quli
    ADVANCED HEALTHCARE MATERIALS, 2018, 7 (15)
  • [37] LIFETIME MAP RECONSTRUCTION IN FREQUENCY-DOMAIN FLUORESCENCE LIFETIME IMAGING MICROSCOPY
    Roudot, Philippe
    Kervrann, Charles
    Waharte, Francois
    Boulanger, Jerome
    2012 IEEE INTERNATIONAL CONFERENCE ON IMAGE PROCESSING (ICIP 2012), 2012, : 2537 - 2540
  • [38] Towards unsupervised fluorescence lifetime imaging using low dimensional variable projection
    Zhang, Yongliang
    Cuyt, Annie
    Lee, Wen-Shin
    Lo Bianco, Giovanni
    Wu, Gang
    Chen, Yu
    Li, David Day-Uei
    OPTICS EXPRESS, 2016, 24 (23): : 26777 - 26791
  • [39] Picosecond fluorescence lifetime imaging microscope for imaging of living glioma cells
    Fang, QY
    Wang, JJ
    Sun, YH
    Vernier, T
    Papaioannou, T
    Jo, J
    Thu, MM
    Gundersen, M
    Marcu, L
    IMAGING, MANIPULATION, AND ANALYSIS OF BIOMOLECULES AND CELLS: FUNDAMENTALS AND APPLICATIONS III, 2005, 5699 : 33 - 39
  • [40] Quantification of integrin receptor agonism by fluorescence lifetime imaging
    Parsons, Maddy
    Messent, Anthea J.
    Humphries, Jonathan D.
    Deakin, Nicholas O.
    Humphries, Martin J.
    JOURNAL OF CELL SCIENCE, 2008, 121 (03) : 265 - 271
12345