Effect of microgravity on gene expression in mouse brain

被引:0
|
作者
Antonio Frigeri
Dumitru A. Iacobas
Sanda Iacobas
Grazia Paola Nicchia
Jean Francois Desaphy
Diana Conte Camerino
Maria Svelto
David C. Spray
机构
[1] University of Bari,Department of General and Environmental Physiology, Centre of Excellence in Comparative Genomics (CEGBA)
[2] Albert Einstein College of Medicine,Department of Neuroscience
[3] University of Bari,Department of Pharmacobiology
来源
Experimental Brain Research | 2008年 / 191卷
关键词
Microgravity; Hindlimb-unloaded; cDNA microarray; Brain; Space flight; Gravitational force; Microgravitational adaptations;
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摘要
Changes in gravitational force such as that experienced by astronauts during space flight induce a redistribution of fluids from the caudad to the cephalad portion of the body together with an elimination of normal head-to-foot hydrostatic pressure gradients. To assess brain gene profile changes associated with microgravity and fluid shift, a large-scale analysis of mRNA expression levels was performed in the brains of 2-week control and hindlimb-unloaded (HU) mice using cDNA microarrays. Although to different extents, all functional categories displayed significantly regulated genes indicating that considerable transcriptomic alterations are induced by HU. Interestingly, the TIC class (transport of small molecules and ions into the cells) had the highest percentage of up-regulated genes, while the most down-regulated genes were those of the JAE class (cell junction, adhesion, extracellular matrix). TIC genes comprised 16% of those whose expression was altered, including sodium channel, nonvoltage-gated 1 beta (Scnn1b), glutamate receptor (Grin1), voltage-dependent anion channel 1 (Vdac1), calcium channel beta 3 subunit (Cacnb3) and others. The analysis performed by GeneMAPP revealed several altered protein classes and functional pathways such as blood coagulation and immune response, learning and memory, ion channels and cell junction. In particular, data indicate that HU causes an alteration in hemostasis which resolves in a shift toward a more hyper-coagulative state with an increased risk of venous thrombosis. Furthermore, HU treatment seems to impact on key steps of synaptic plasticity and learning processes.
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页码:289 / 300
页数:11
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