Comparison of raw and processed Radix Polygoni Multiflori (Heshouwu) by high performance liquid chromatography and mass spectrometry

被引：46

作者：

Liang Z. ^{[1
]}

Chen H. ^{[1
]}

Yu Z. ^{[1
]}

Zhao Z. ^{[1
]}

机构：

[1] School of Chinese Medicine, Hong Kong Baptist University, Kowloon, Hong Kong SAR

来源：

Chinese Medicine | / 5卷 / 1期

关键词：

High Performance Liquid Chromatography; Glucoside; Anthraquinone; Chinese Pharmacopoeia; High Performance Liquid Chromatography Condition;

D O I：

10.1186/1749-8546-5-29

中图分类号：

学科分类号：

摘要：

Background: Radix Polygoni Multiflori is the dried root tuber of Polygonum multiflorum Thunb. (Fam. Polygonaceae). According to Chinese medicine theory, raw (R-RPM) and processed (P-RPM) Radix Polygoni Multiflori possess different properties. The present study investigates the differences in chemistry between raw and processed Radix Polygoni Multiflori.Methods: Five pairs of R-RPM and P-RPM as well as 15 commercial decoction pieces were analyzed with high performance liquid chromatography (HPLC) and mass spectrometry (MS).Results: Two anthraquinones, namely emodin-8-O-(6'-O-malonyl)-glucoside and physcion-8-O-(6'-O-malonyl)-glucoside disappeared or decreased significantly and 2,3,5,4'-tetrahydroxystilbene-2-O-β-D-glucopyranoside, emodin-8-O-β-D-glucopyranoside and physcion-8-O-β-D-glucopyranoside decreased after the R-RPM samples being processed. On the other hand, the contents of emodin and physcion generally increased after processing.Conclusion: The present study indicates that processing Radix Polygoni Multiflori may change the contents and types of chemicals in it. These changes are probably responsible for the various pharmacological effects of R-RPM and P-RPM as well as hepatotoxicity. © 2010 Liang et al; licensee BioMed Central Ltd.

引用

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