Properties of plasma membrane H+-ATPase in salt-treated Populus euphratica callus

被引：0
|
作者：
Yingli Yang
Feng Zhang
Meigui Zhao
Lizhe An
Lixin Zhang
Nianlai Chen
机构：
[1] Northwest Normal University,School of Life Science
[2] Chinese Academy of Sciences,Cold and Arid Regions Environmental and Engineering Research Institute
[3] Chinese Academy of Sciences,Institute of Botany
来源：
Plant Cell Reports | 2007年 / 26卷
关键词：
H; -ATPase; NaCl; Plasma membrane; callus; Thiol group;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
The plasma membrane (PM) vesicles from Populus euphratica (P. euphratica) callus were isolated to investigate the properties of the PM H+-ATPase. An enrichment of sealed and oriented right-side-out PM vesicles was demonstrated by measurement of the purity and orientation of membrane vesicles in the upper phase fraction. Analysis of pH optimum, temperature effects and kinetic properties showed that the properties of the PM H+-ATPase from woody plant P. euphratica callus were consistent with those from herbaceous species. Application of various thiol reagents to the reaction revealed that reduced thiol groups were essential to maintain the PM H+-ATPase activity. In addition, there was increased H+-ATPase activity in the PM vesicles when callus was exposed to NaCl. Western blotting analysis demonstrated an enhancement of H+-ATPase content in NaCl-treated P. euphratica callus compared with the control.
引用
收藏
相关论文
共 50 条
  • [1] Properties of plasma membrane H+-ATPase in salt-treated Populus euphratica callus
    Yang, Yingli
    Zhang, Feng
    Zhao, Meigui
    An, Lizhe
    Zhang, Lixin
    Chen, Nianlai
    PLANT CELL REPORTS, 2007, 26 (02) : 229 - 235
  • [2] Study on purification and activity of plasma membrane H+-ATPase of Populus euphratica.
    Zhang, L
    Liu, QL
    Huang, YG
    PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS, 2000, 27 (06) : 637 - 640
  • [3] Cloning of plasma membrane H+-ATPase gene in Populus euphratica Oliv.
    Ning De-juan Hou Pei-chen Hu Zan-min Shen Xin Chen Shao-liang College of Biological Sciences and Biotechnology
    ForestryStudiesinChina, 2006, (04) : 15 - 19
  • [4] Tonoplast H+-ATPase in response to salt stress in Populus euphratica cell suspensions
    Ma, TJ
    Liu, QL
    Li, Z
    Zhang, XJ
    PLANT SCIENCE, 2002, 163 (03) : 499 - 505
  • [5] Studies on the proton pumping activity of H+-ATPase in tonoplast vesicles of Populus euphratica
    Liu, QL
    Zhang, XJ
    Li, Y
    Wang, SS
    Jiang, XN
    ACTA BOTANICA SINICA, 2001, 43 (05): : 495 - 500
  • [6] Effects of freezing on plasma membrane H+-ATPase of the callus from Chorispora bungeana
    Wu, J. M.
    Zhao, Z. G.
    Xing, H.
    Guo, H. P.
    Li, W. X.
    An, L. Z.
    Xu, S. J.
    Chen, T.
    BIOLOGIA PLANTARUM, 2007, 51 (02) : 229 - 234
  • [7] Populus euphratica WRKY1 binds the promoter of H+-ATPase gene to enhance gene expression and salt tolerance
    Yao, Jun
    Shen, Zedan
    Zhang, Yanli
    Wu, Xia
    Wang, Jianhui
    Sa, Gang
    Zhang, Yuhong
    Zhang, Huilong
    Deng, Chen
    Liu, Jian
    Hou, Siyuan
    Zhang, Ying
    Zhang, Yinan
    Zhao, Nan
    Deng, Shurong
    Lin, Shanzhi
    Zhao, Rui
    Chen, Shaoliang
    JOURNAL OF EXPERIMENTAL BOTANY, 2020, 71 (04) : 1527 - 1539
  • [8] Relationship between expression of the PM H+-ATPase, growth and ion partitioning in the leaves of salt-treated Medicago species
    Sibole, JV
    Cabot, C
    Michalke, W
    Poschenrieder, C
    Barceló, J
    PLANTA, 2005, 221 (04) : 557 - 566
  • [9] Isolation of protoplast from callus of Populus euphratica and H~+ fluxes across plasma membrane under NaCl stress
    Gao Zhun1 Dai Song-xiang1 Chen Shao-liang1
    Forestry Studies in China, 2007, (03) : 198 - 202
  • [10] Relationship between expression of the PM H+-ATPase, growth and ion partitioning in the leaves of salt-treated Medicago species
    John V. Sibole
    Catalina Cabot
    Wolfgang Michalke
    Charlotte Poschenrieder
    Juan Barceló
    Planta, 2005, 221 : 557 - 566
12345