Properties of plasma membrane H+-ATPase in salt-treated Populus euphratica callus

被引：0
作者：
Yingli Yang
Feng Zhang
Meigui Zhao
Lizhe An
Lixin Zhang
Nianlai Chen
机构：
[1] Northwest Normal University,School of Life Science
[2] Chinese Academy of Sciences,Cold and Arid Regions Environmental and Engineering Research Institute
[3] Chinese Academy of Sciences,Institute of Botany
来源：
Plant Cell Reports | 2007年 / 26卷
关键词：
H; -ATPase; NaCl; Plasma membrane; callus; Thiol group;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
The plasma membrane (PM) vesicles from Populus euphratica (P. euphratica) callus were isolated to investigate the properties of the PM H+-ATPase. An enrichment of sealed and oriented right-side-out PM vesicles was demonstrated by measurement of the purity and orientation of membrane vesicles in the upper phase fraction. Analysis of pH optimum, temperature effects and kinetic properties showed that the properties of the PM H+-ATPase from woody plant P. euphratica callus were consistent with those from herbaceous species. Application of various thiol reagents to the reaction revealed that reduced thiol groups were essential to maintain the PM H+-ATPase activity. In addition, there was increased H+-ATPase activity in the PM vesicles when callus was exposed to NaCl. Western blotting analysis demonstrated an enhancement of H+-ATPase content in NaCl-treated P. euphratica callus compared with the control.
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