Expression and localization of human lysozyme in the endosperm of transgenic rice

被引：0

作者：

Daichang Yang

Fengli Guo

Bo Liu

Ning Huang

Simon C. Watkins

机构：

[1] Ventria Bioscience,

[2] 4110 North Freeway,undefined

[3] Sacramento,undefined

[4] CA 95834,undefined

[5] USA,undefined

[6] Section of Plant Biology,undefined

[7] University of California,undefined

[8] Davis,undefined

[9] CA 95616,undefined

[10] USA,undefined

[11] Department of Cell Biology and Physiology,undefined

[12] University of Pittsburgh,undefined

[13] PA 15261,undefined

[14] USA,undefined

来源：

Planta | 2003年 / 216卷

关键词：

Lysozyme (human) Oryza Signal peptide Storage-protein targeting Transgenic rice;

D O I：

暂无

中图分类号：

学科分类号：

摘要：

In order to understand the characteristics of recombinant protein expression and sublocalization in rice (Oryza sativa L.) endosperm, we examined the expression level of human lysozyme protein and its subcellular location in transgenic rice seeds driven by rice glutelin and globulin promoters and signal peptides. A time course of human lysozyme expression during endosperm development was analyzed. The results showed that the expression profile of recombinant protein accumulation in endosperm paralleled that of the two storage proteins. Immunofluorescence microscopy revealed that human lysozyme and storage proteins co-localized to type-II protein bodies. Both promoter–signal peptide parings targeted recombinant protein to the protein bodies. In addition, a transgenic line with a higher lysozyme expression level exhibited morphologically different protein bodies with an unbalanced composition of lysozyme and native storage proteins. The high-level expression of recombinant protein distorted the trafficking and sorting of native storage proteins in rice endosperm and affected the expression of native storage protein.

引用

页码：597 / 603

页数：6