Genetic linkage map of melon (Cucumis melo L.) and localization of a major QTL for powdery mildew resistance

被引：0
作者：
Fernando J. Yuste-Lisbona
Carmen Capel
Emilio Sarria
Rocío Torreblanca
María L. Gómez-Guillamón
Juan Capel
Rafael Lozano
Ana I. López-Sesé
机构：
[1] Consejo Superior de Investigaciones Científicas (CSIC),Departamento de Mejora Vegetal, Estación Experimental ‘La Mayora’ (EELM)
[2] Universidad de Almería,Departamento de Biología Aplicada (Genética), Edificio CITE II
来源：
Molecular Breeding | 2011年 / 27卷
关键词：
Codominant DNA markers; Disease resistance; Quantitative trait loci;
D O I：
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摘要：
Powdery mildew caused by Podosphaera xanthii has become a major problem in melon since it occurs all year round irrespective of the growing system. The TGR-1551 melon genotype was found to be resistant to several melon diseases, among them powdery mildew. However, the corresponding resistance genes have been never mapped. We constructed an integrated genetic linkage map using an F2 population derived from a cross between the multi-resistant genotype TGR-1551 and the susceptible Spanish cultivar ‘Bola de Oro’. The map spans 1,284.9 cM, with an average distance of 3.6 cM among markers, and consists of 354 loci (188 AFLP, 39 RAPD, 111 SSR, 14 SCAR/CAPS/dCAPS, and two phenotypic traits) distributed in 14 linkage groups. QTL analysis identified one major QTL (Pm-R) on LG V for resistance to races 1, 2, and 5 of powdery mildew. The PM4-CAPS marker is closely linked to the Pm-R QTL at a genetic distance of 1.9 cM, and the PM3-CAPS marker is located within the support interval of this QTL. These codominant markers, together with the map information reported here, could be used for melon breeding, and particularly for genotyping selection of resistance to powdery mildew in this vegetable crop species.
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页码：181 / 192
页数：11
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