Essential validation methods for E. coli strains created by chromosome engineering

[1] Lee EC(2001)A Highly Efficient Escherichia coli-Based Chromosome Engineering System Adapted for Recombinogenic Targeting and Subcloning of BAC DNA Genomics 73 56-65

[2] Yu D(1997)The Complete Genome Sequence of Escherichia coli K-12 Science 277 1453-62

[3] Martinez de Velasco J(2001)High efficiency mutagenesis, repair, and engineering of chromosomal DNA using single-stranded oligonucleotides Proceedings of the National Academy of Sciences 98 6742-6

[4] Tessarollo L(2000)Towards Single-Copy Gene Expression Systems Making Gene Cloning Physiologically Relevant: Lambda InCh, a Simple Escherichia coli Plasmid-Chromosome Shuttle System Journal of Bacteriology 182 842-7

[5] Swing DA(2010)Stoichiometry and Architecture of Active DNA Replication Machinery in Escherichia coli Science 328 498-501

[6] Court DL(2008)Independent Positioning and Action of Escherichia coli Replisomes in Live Cells Cell 133 90-102

[7] Blattner FR(2012)Superresolution imaging of ribosomes and RNA polymerase in live Escherichia coli cells Molecular Microbiology 85 21-38

[8] Plunkett G(2006)Complete set of ORF clones of Escherichia coli ASKA library (A Complete Set of E. coli K-12 ORF Archive): Unique Resources for Biological Research DNA Research 12 291-9

[9] Bloch CA(2003)Spatial and temporal organization of replicating Escherichia coli chromosomes Molecular Microbiology 49 731-43

[10] Perna NT(2009)Self-Organization of the Escherichia coli Chemotaxis Network Imaged with Super-Resolution Light Microscopy PLoS Biol 7 e1000137-29

[1] Lee EC(2001)A Highly Efficient Escherichia coli-Based Chromosome Engineering System Adapted for Recombinogenic Targeting and Subcloning of BAC DNA Genomics 73 56-65

[2] Yu D(1997)The Complete Genome Sequence of Escherichia coli K-12 Science 277 1453-62

[3] Martinez de Velasco J(2001)High efficiency mutagenesis, repair, and engineering of chromosomal DNA using single-stranded oligonucleotides Proceedings of the National Academy of Sciences 98 6742-6

[4] Tessarollo L(2000)Towards Single-Copy Gene Expression Systems Making Gene Cloning Physiologically Relevant: Lambda InCh, a Simple Escherichia coli Plasmid-Chromosome Shuttle System Journal of Bacteriology 182 842-7

[5] Swing DA(2010)Stoichiometry and Architecture of Active DNA Replication Machinery in Escherichia coli Science 328 498-501

[6] Court DL(2008)Independent Positioning and Action of Escherichia coli Replisomes in Live Cells Cell 133 90-102

[7] Blattner FR(2012)Superresolution imaging of ribosomes and RNA polymerase in live Escherichia coli cells Molecular Microbiology 85 21-38

[8] Plunkett G(2006)Complete set of ORF clones of Escherichia coli ASKA library (A Complete Set of E. coli K-12 ORF Archive): Unique Resources for Biological Research DNA Research 12 291-9

[9] Bloch CA(2003)Spatial and temporal organization of replicating Escherichia coli chromosomes Molecular Microbiology 49 731-43

[10] Perna NT(2009)Self-Organization of the Escherichia coli Chemotaxis Network Imaged with Super-Resolution Light Microscopy PLoS Biol 7 e1000137-29