Translocator Protein Is Involved in the Induction and Regulation of Mitochondrial Permeability Transition Pore Opening in C6 Glioma Cells

被引：0

作者：

Baburina Y.L. ^{[1
]}

Sotnikova L.D. ^{[1
]}

Krestinina O.V. ^{[1
]}

机构：

[1] Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Moscow oblast, Pushchino

来源：

Cell and Tissue Biology | 2023年 / 17卷 / 2期

关键词：

C6 glioma cells; mitochondria; mitochondrial transition permeability pore; oxidative stress; protoporphyrin IX; translocator protein;

D O I：

10.1134/S1990519X23020037

中图分类号：

学科分类号：

摘要：

Abstract: Translocator protein (TSPO), formerly known as the peripheral-type benzodiazepine receptor (PBR), is a 18-kDa protein localized in the outer mitochondrial membrane and involved in cell proliferation and apoptosis. In the present work, we found TSPO as a monomer and dimer in rat C6 glioma cells. We have shown that a reduced TSPO expression in cells affects the induction of a mitochondrial permeability transition pore (mPTP). It also stimulated the Ca2+-induced opening of mPTP. The natural endogenous TSPO ligand protoporphyrin IX (PPIX) accelerated the opening of mPTP. The stimulatory effect of PPIX was higher in the mitochondria of wild-type C6 glioma cells than in TSPO knockdown C6 glioma cells. Phosphorylation of membrane receptors, ion channels, and transcription factors is an important intracellular signaling event responsible for the regulation of various enzymes and many cellular functions. In the present study, PPIX modulated protein phosphorylation. We hypothesized that TSPO is an important protein in the regulation of cell survival and may be involved in mPTP functioning. © 2023, Pleiades Publishing, Ltd.

引用

页码：179 / 187

页数：8

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