A modified mini-prep method for economical and rapid extraction of genomic DNA in plants

被引：35

作者：

Khan I.A. ^{[1
]}

Awan F.S. ^{[1
]}

Ahmad A. ^{[1
]}

Khan A.A. ^{[1
]}

机构：

[1] Centre of Agricultural Biochemistry and Biotechnology (CABB), University of Agriculture Faisalabad

来源：

Plant Molecular Biology Reporter | 2004年 / 22卷 / 1期

关键词：

Cotton; DNA extraction method; Molecular markers; Wheat;

D O I：

10.1007/BF02773355

中图分类号：

学科分类号：

摘要：

Molecular markers for map-based cloning, marker-assisted selection in crop breeding, and genetic studies require DNA isolation from a large number of plants in a short span of time. Here we describe a modified DNA extraction method that is economical in terms of cost, time, and labour. The method allows DNA extraction from as little as 0.2-0.3 g of leaves that are homogenized in zipper plastic bags, followed by DNA isolation in 1.5-mL Eppendorf tubes. By using the modified method, a DNA yield of 700-800 μg/300 mg leaf tissue was obtained from cotton and wheat samples. The quality of the DNA was quite suitable for PCR-based markers. © 2004 International Society for Plant Molecular Biology.

引用

页码：89a / 89e

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