Formation of Tissue-Engineered Construct of Human Cartilage Tissue in a Flow-Through Bioreactor

被引：0
|
作者：
V. I. Sevastianov
Yu. B. Basok
A. M. Grigor’ev
L. A. Kirsanova
V. N. Vasilets
机构：
[1] Ministry of Health of the Russian Federation,V. I. Shumakov Federal Research Center of Transplantology and Artificial Organs
来源：
Bulletin of Experimental Biology and Medicine | 2017年 / 164卷
关键词：
flow-through bioreactor; cell- and tissue-engineered constructs; biopolymer matrix; mesenchymal stromal cells; cartilage tissue;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
We performed culturing of a cell-engineered construct of human cartilage tissue consisting of biopolymer microstructured collagen-containing hydrogel, human adipose tissue mesenchymal stromal cells, and induction chondrogenic culture medium in a specially designed flow-through bioreactor. On day 16 of the experiment, human adipose tissue mesenchymal stromal cells acquired flattened shape typical for chondroblasts, demonstrated high proliferative activity, and formed extracellular matrix. The observed histological changes in the cultured system attested to the beginning of the formation of a tissue-engineered construct of human cartilage tissue.
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页码：269 / 273
页数：4
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