Effect of the concentration of suspended solids on the enzymatic activities and biodiversity of a submerged membrane bioreactor for aerobic treatment of domestic wastewater

被引：0
作者：
M. Molina-Muñoz
J. M. Poyatos
R. Vílchez
E. Hontoria
B. Rodelas
J. González-López
机构：
[1] University of Granada,Institute of Water Research
[2] University of Granada,Department of Civil Engineering
[3] University of Granada,Department of Microbiology
来源：
Applied Microbiology and Biotechnology | 2007年 / 73卷
关键词：
Submerged membrane bioreactor; Domestic wastewater; Wastewater treatment; Biodiversity; Enzymatic activities; TGGE;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
A pilot-scale submerged membrane bioreactor was used for the treatment of domestic wastewater in order to study the influence of the variations in the concentration of volatile suspended solids (VSS) on the enzymatic activities (acid and alkaline phosphatases, glucosidase, protease, esterase, and dehydrogenase) and biodiversity of the bacterial community in the sludge. The influence of VSS concentration was evaluated in two separated experiments, which were carried out in two different seasons of the year (experiment 1 through spring–summer and experiment 2 through autumn–winter). Cluster analysis of the temperature gradient gel electrophoresis (TGGE) profiles demonstrated that the community composition was significantly different in both experiments. Within the same experiment, the bacterial community experienced sequential shifts as the biomass accumulated, as shown by the evolution of the population profiles through time as VSS concentration increased. All enzymatic activities studied were significantly lower during experiment 2, except for glucosidase. Concentrations of VSS over 8 g/l induced a strong descent of all enzymatic activities, which overlapped with a significant modification of the community composition. Sequences of the major TGGE bands were identified as representatives of the Alpha-proteobacteria, filamentous bacteria (Thiotrix), and nitrite oxidizers (Nitrospira). Some sequences which were poorly related to any validated bacterial taxon were obtained.
引用
收藏
页码：1441 / 1451
页数：10
相关论文
共 133 条
[1]  
Altschul SF(1997)Gapped BLAST and PSI-BLAST: a new generation of protein database search programs Nucleic Acids Res 25 3389-3402
[2]  
Madden TL(1998)Optimisation of sludge acidogenic fermentation for biological nutrient removal Water SA 24 35-41
[3]  
Schaeffer AA(2001)Characterization and distribution of esterase activity in activated sludge Water Res 35 4208-4216
[4]  
Zhang J(2000)Competitive PCR-DGGE analysis of bacterial mixtures: an internal standard and an appraisal of template enumeration accuracy J Microbiol Methods 40 111-123
[5]  
Zhang Z(2002)Availability of low and high molecular weight substrates to extracellular enzymes in whole and dispersed activated sludged Enzyme Microb Technol 31 179-186
[6]  
Miller W(2003)A review of membrane bioreactors and their potential application in the treatment of agricultural wastewater Can Biosyst Eng 45 637-649
[7]  
Lipman DJ(2006)Analysis of community composition of biofilms in a submerged filter system for the removal of ammonia and phenol from an industrial wastewater Biochem Soc Trans 34 165-168
[8]  
Banister SS(2003)Community analysis of ammonia and nitrite oxidizers during start-up of nitritation reactors Appl Environ Microbiol 69 3213-3222
[9]  
Pretorius WA(2003)Lipase and protease extraction from activated sludge Water Res 37 3652-3657
[10]  
Boczar B(1998)Enzyme activities under anaeroic conditions in activated sludge sequencing batch reactor Water Res 32 2081-2088
12345678910