Quantitative multiplex real-time polymerase chain reaction assay for the detection of Helicobacter pylori and clarithromycin resistance (vol 23, 155, 2023)

被引：0

作者：

Kim, Ilsoo ^{[1
]}

Maeng, Lee-So ^{[2
]}

Kim, Joon Sung ^{[1
]}

Kim, Byung-Wook ^{[1
]}

Cheung, Dae Young ^{[3
]}

Kim, Jin Il ^{[3
]}

Park, Soo-heon ^{[3
]}

机构：

[1] Catholic Univ Korea, Incheon St Marys Hosp, Coll Med, Dept Internal Med,Div Gastroenterol, Seoul, South Korea

[2] Catholic Univ Korea, Dept Hosp Pathol, Incheon St Marys Hosp, Coll Med, Seoul, South Korea

[3] Catholic Univ Korea, Yeouido St Marys Hosp, Coll Med, Dept Internal Med, Seoul, South Korea

来源：

BMC MICROBIOLOGY | 2023年 / 23卷 / 01期

关键词：

Helicobacter Pylori; Macrolide Resistance; Molecular diagnosis;

D O I：

10.1186/s12866-023-02937-3

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Background: Identifying clarithromycin resistance is essential for eradicating Helicobacter pylori (HP). Therefore, we evaluated the performance of Allplex™ H.pylori & ClariR Assay (Allplex™) for diagnosing and detecting clarithromycin resistance in HP. Methods: Subjects who underwent esophagogastroduodenoscopy between April 2020 and August 2021 at Incheon St. Mary’s hospital were enrolled in this study. The diagnostic performances of Allplex™ and dual priming oligonucleotide (DPO)-based multiplex polymerase chain reaction (PCR) were compared with sequencing as the gold standard. Results: A total of 142 gastric biopsy samples were analyzed. Gene sequencing revealed 124 HP infections, 42 A2143G mutations, 2 A2142G mutations, one dual mutation, and no A2142C mutation. DPO-PCR showed 96.0% sensitivity and 100.0% specificity for HP detection; the corresponding rates for Allplex™ were 99.2% and 100.0%. DPO-PCR showed 88.3% sensitivity and 82.0% specificity for A2143G mutation, and Allplex™ showed 97.6% and 96.0%. The Cohen’s Kappa coefficient for overall test results was 0.56 for DPO-PCR and 0.95 for Allplex™. Conclusion: Allplex™ showed comparable diagnostic performance with direct gene sequencing and non-inferior diagnostic performance to DPO-PCR. Further research is required to confirm whether Allplex™ is an effective diagnostic tool for the eradication of HP. © 2023, The Author(s).

引用

页数：1