Introducing a New Model of Sweet Taste Receptor, a Class C G-protein Coupled Receptor (C GPCR)
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作者:
Elaheh Kashani-Amin
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机构:Tehran University of Medical Sciences,Biosensor Research Center, Endocrinology and Metabolism Molecular
Elaheh Kashani-Amin
Amirhossein Sakhteman
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机构:Tehran University of Medical Sciences,Biosensor Research Center, Endocrinology and Metabolism Molecular
Amirhossein Sakhteman
Bagher Larijani
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机构:Tehran University of Medical Sciences,Biosensor Research Center, Endocrinology and Metabolism Molecular
Bagher Larijani
Azadeh Ebrahim-Habibi
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机构:Tehran University of Medical Sciences,Biosensor Research Center, Endocrinology and Metabolism Molecular
Azadeh Ebrahim-Habibi
机构:
[1] Tehran University of Medical Sciences,Biosensor Research Center, Endocrinology and Metabolism Molecular
[2] Shiraz University of Medical Sciences,Cellular Sciences Institute
[3] Shiraz University of Medical Sciences,Department of Medicinal Chemistry, School of Pharmacy
[4] Tehran University of Medical Sciences,Medicinal Chemistry and Natural Products Research Center
来源:
Cell Biochemistry and Biophysics
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2019年
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77卷
关键词:
Sweet taste receptor;
T1R2;
T1R3;
C GPCR;
Homology modeling;
D O I:
暂无
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学科分类号:
摘要:
The structure of sweet taste receptor (STR), a heterodimer of class C G-protein coupled receptors comprising T1R2 and T1R3 molecules, is still undetermined. In this study, a new enhanced model of the receptor is introduced based on the most recent templates. The improvement, stability, and reliability of the model are discussed in details. Each domain of the protein, i.e., VFTM, CR, and TMD, were separately constructed by hybrid-model construction methods and then assembled to build whole monomers. Overall, 680 ns molecular dynamics simulation was performed for the individual domains, the whole monomers and the heterodimer form of the VFTM orthosteric binding site. The latter’s structure obtained from 200 ns simulation was docked with aspartame; among various binding sites suggested by FTMAP server, the experimentally suggested binding domain in T1R2 was retrieved. Local three-dimensional structures and helices spans were evaluated and showed acceptable accordance with the template structures and secondary structure predictions. Individual domains and whole monomer structures were found stable and reliable to be used. In conclusion, several validations have shown reliability of the new and enhanced models for further molecular modeling studies on structure and function of STR and C GPCRs.
机构:
Univ Tehran Med Sci, Biosensor Res Ctr, Endocrinol & Metab Mol Cellular Sci Inst, Tehran, IranUniv Tehran Med Sci, Biosensor Res Ctr, Endocrinol & Metab Mol Cellular Sci Inst, Tehran, Iran
Kashani-Amin, Elaheh
Sakhteman, Amirhossein
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机构:
Shiraz Univ Med Sci, Sch Pharm, Dept Med Chem, Shiraz, Iran
Shiraz Univ Med Sci, Med Chem & Nat Prod Res Ctr, Shiraz, IranUniv Tehran Med Sci, Biosensor Res Ctr, Endocrinol & Metab Mol Cellular Sci Inst, Tehran, Iran
Sakhteman, Amirhossein
Larijani, Bagher
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机构:
Univ Tehran Med Sci, Endocrinol & Metab Res Ctr, Endocrinol & Metab Clin Sci Inst, Tehran, IranUniv Tehran Med Sci, Biosensor Res Ctr, Endocrinol & Metab Mol Cellular Sci Inst, Tehran, Iran
机构:
Univ Tehran Med Sci, Biosensor Res Ctr, Endocrinol & Metab Mol Cellular Sci Inst, Tehran, IranUniv Tehran Med Sci, Biosensor Res Ctr, Endocrinol & Metab Mol Cellular Sci Inst, Tehran, Iran
Kashani-Amin, Elaheh
Sakhteman, Amirhossein
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h-index: 0
机构:
Shiraz Univ Med Sci, Sch Pharm, Dept Med Chem, Shiraz, Iran
Shiraz Univ Med Sci, Med Chem & Nat Prod Res Ctr, Shiraz, IranUniv Tehran Med Sci, Biosensor Res Ctr, Endocrinol & Metab Mol Cellular Sci Inst, Tehran, Iran
Sakhteman, Amirhossein
Larijani, Bagher
论文数: 0引用数: 0
h-index: 0
机构:
Univ Tehran Med Sci, Endocrinol & Metab Res Ctr, Endocrinol & Metab Clin Sci Inst, Tehran, IranUniv Tehran Med Sci, Biosensor Res Ctr, Endocrinol & Metab Mol Cellular Sci Inst, Tehran, Iran
机构:
Univ Illinois, Dept Biochem, 315 Roger Adams Lab,600 South Mathews Ave, Urbana, IL 61801 USAUniv Illinois, Dept Biochem, 315 Roger Adams Lab,600 South Mathews Ave, Urbana, IL 61801 USA
Park, Jihye
Selvam, Balaji
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机构:
Univ Illinois, Dept Chem & Biomol Engn, Urbana, IL 61801 USAUniv Illinois, Dept Biochem, 315 Roger Adams Lab,600 South Mathews Ave, Urbana, IL 61801 USA
Selvam, Balaji
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Sanematsu, Keisuke
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Shigemura, Noriatsu
Shukla, Diwakar
论文数: 0引用数: 0
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机构:
Univ Illinois, Dept Chem & Biomol Engn, Urbana, IL 61801 USAUniv Illinois, Dept Biochem, 315 Roger Adams Lab,600 South Mathews Ave, Urbana, IL 61801 USA
Shukla, Diwakar
Procko, Erik
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Univ Illinois, Dept Biochem, 315 Roger Adams Lab,600 South Mathews Ave, Urbana, IL 61801 USAUniv Illinois, Dept Biochem, 315 Roger Adams Lab,600 South Mathews Ave, Urbana, IL 61801 USA
机构:
Univ Calif Berkeley, Dept Mol & Cell Biol, 229 Stanley Hall, Berkeley, CA 94720 USAUniv Calif Berkeley, Dept Mol & Cell Biol, 229 Stanley Hall, Berkeley, CA 94720 USA
Vafabakhsh, Reza
Levitz, Joshua
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Univ Calif Berkeley, Dept Mol & Cell Biol, 229 Stanley Hall, Berkeley, CA 94720 USAUniv Calif Berkeley, Dept Mol & Cell Biol, 229 Stanley Hall, Berkeley, CA 94720 USA
Levitz, Joshua
Isacoff, Ehud Y.
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Univ Calif Berkeley, Dept Mol & Cell Biol, 229 Stanley Hall, Berkeley, CA 94720 USA
Univ Calif Berkeley, Helen Wills Neurosci Inst, Berkeley, CA 94720 USA
Univ Calif Berkeley, Lawrence Berkeley Natl Lab, Phys Biosci Div, Berkeley, CA 94720 USAUniv Calif Berkeley, Dept Mol & Cell Biol, 229 Stanley Hall, Berkeley, CA 94720 USA