A germline-competent embryonic stem cell line from NOD.Cg-PrkdcscidIl2rgtm1Wjl/SzJ (NSG) mice

被引：0
作者：
Carlisle P. Landel
Jennifer Dunlap
John B. Patton
Tim Manser
机构：
[1] Thomas Jefferson University,Department of Microbiology and Immunology
[2] Thomas Jefferson University,The Kimmel Cancer Center
[3] Transposagen Biopharmaceuticals Inc,undefined
来源：
Transgenic Research | 2013年 / 22卷
关键词：
NSG mice; ES cells; Humanized mice;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
The NOD.Cg-PrkdcscidIl2rgtm1Wjl/SzJ mouse strain, commonly known as NSG (for NOD SCID Gamma) is severely immunodeficient and thus is an excellent recipient for xenografts, and in particular for engrafting human tumor cells and human hematopoietic stem cells. In the latter case, these cells give rise to many human hematopoetic lineages in their NSG hosts, resulting in recapitulation of many of the features of a human immune system. However, the immune system of these “humanized mice” (huMice) is not completely functional, in part because of a lack of expression of necessary human cytokines and HLA molecules by NSG host tissues. In order to facilitate the genetic modification of this strain in order to improve the huMouse model, we have created germline competent ES cells of this strain in which such modifications can be carried out.
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页码：179 / 185
页数：6
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