Kinetics of hydrogen peroxide decomposition by catalase: hydroxylic solvent effects

被引：0
作者：
Adina Raducan
Anca Ruxandra Cantemir
Mihaela Puiu
Dumitru Oancea
机构：
[1] University of Bucharest,Department of Physical Chemistry, Faculty of Chemistry
[2] LaborQ,undefined
[3] University of Bucharest,undefined
来源：
Bioprocess and Biosystems Engineering | 2012年 / 35卷
关键词：
Catalase kinetics; Hydrogen peroxide decomposition; Solvent effects;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
The effect of water–alcohol (methanol, ethanol, propan-1-ol, propan-2-ol, ethane-1,2-diol and propane-1,2,3-triol) binary mixtures on the kinetics of hydrogen peroxide decomposition in the presence of bovine liver catalase is investigated. In all solvents, the activity of catalase is smaller than in water. The results are discussed on the basis of a simple kinetic model. The kinetic constants for product formation through enzyme–substrate complex decomposition and for inactivation of catalase are estimated. The organic solvents are characterized by several physical properties: dielectric constant (D), hydrophobicity (log P), concentration of hydroxyl groups ([OH]), polarizability (α), Kamlet-Taft parameter (β) and Kosower parameter (Z). The relationships between the initial rate, kinetic constants and medium properties are analyzed by linear and multiple linear regression.
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页码：1523 / 1530
页数：7
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