LaeA regulates morphological development and ochratoxin A biosynthesis in Aspergillus niger

被引：0
作者：
Mengwei Zhang
Yan Yang
Linlin Li
Shuwen Liu
Xianli Xue
Qiang Gao
Depei Wang
Ying Zhang
Jian Zhang
机构：
[1] Tianjin University of Science and Technology,College of Biotechnology
[2] Tianjin University of Science and Technology,Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education
[3] Tianjin University of Science and Technology,College of Food Science and Engineering
来源：
Mycotoxin Research | 2022年 / 38卷
关键词：
LaeA; Morphological development; Ochratoxin A; Gene expression;
D O I：
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中图分类号：
学科分类号：
摘要：
The global regulator LaeA and its orthologs govern the morphogenetic development and secondary metabolism of several filamentous ascomycetes. In Aspergillus niger, it has been shown that an LaeA ortholog (AnLaeA) regulates the production of citric acid and secondary metabolites. In this work, we constructed AnlaeA disruption and overexpression strains to investigate the roles of AnLaeA in morphological development and ochratoxin A (OTA) biosynthesis in A. niger. Phenotypic observation, chemical analysis, and gene expression analysis indicated that AnLaeA acts as a negative regulator of conidial morphogenesis and positively regulates gene expression of the OTA cluster in A. niger grown in CYA medium. However, it was observed that the upregulation of gene expression of the OTA cluster does not necessarily increase OTA production. Our results contribute to a better understanding of the AnlaeA regulatory mechanism and suggest the AnlaeA gene as a potential target for developing control strategies for A. niger infection and OTA biosynthesis.
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页码：221 / 229
页数：8
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