Isolation, characterization and molecular cloning of a leaf-specific lectin from ramsons (Allium ursinum L.).

被引:0
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作者
Koen Smeets
Els J.M. Van Damme
Fred Van Leuven
Willy J. Peumans
机构
[1] Katholieke Universiteit Leuven,Laboratory for Phytopathology and Plant Protection
[2] Katholieke Universiteit Leuven,Center for Human Genetics
来源
Plant Molecular Biology | 1997年 / 35卷
关键词
Allium/; leaf-specific; lectin; ramsons;
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摘要
Lectins were isolated from roots and leaves of ramsons and compared to the previously described bulb lectins. Biochemical analyses indicated that the root lectins AUAIr and AUAIIr are identical to the bulb lectins AUAI and AUAII, whereas the leaf lectin AUAL has no counterpart in the bulbs. cDNA cloning confirmed that the leaf lectin differs from the bulb lectins. Northern blot analysis further indicated that the leaf lectin is tissue-specifically expressed. Sequence comparisons revealed that the ramsons leaf lectin differs considerably from the leaf lectins of garlic, leek, onion and shallot.
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页码:531 / 535
页数:4
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