Isolation and cultivation of stem cells from adult mouse testes

被引：0
作者：
Kaomei Guan
Frieder Wolf
Alexander Becker
Wolfgang Engel
Karim Nayernia
Gerd Hasenfuss
机构：
[1] Heart Center,Department of Cardiology and Pneumology
[2] Georg-August-University of Göttingen,undefined
[3] Institute of Human Genetics,undefined
[4] Georg-August-University of Göttingen,undefined
[5] Present address: Institute of Human Genetics,undefined
[6] University of Newcastle upon Tyne,undefined
[7] International Centre for Life,undefined
[8] Central Parkway,undefined
[9] Newcastle upon Tyne NE1 3BZ,undefined
[10] UK.,undefined
来源：
Nature Protocols | 2009年 / 4卷
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摘要：
The successful isolation and cultivation of spermatogonial stem cells (SSCs) as well as induction of SSCs into pluripotent stem cells will allow us to study their biological characteristics and their applications in therapeutic approaches. Here we provide step-by-step procedures on the basis of previous work in our laboratory for: the isolation of testicular cells from adolescent mice by a modified enzymatic procedure; the enrichment of undifferentiated spermatogonia by laminin selection or genetic selection using Stra8-EGFP (enhanced green fluorescent protein) transgenic mice; the cultivation and conversion of undifferentiated spermatogonia into embryonic stem-like cells, so-called multipotent adult germline stem cells (maGSCs); and characterization of these cells. Normally, it will take about 16 weeks to obtain stable maGSC lines starting from the isolation of testicular cells.
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页码：143 / 154
页数：11
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