Effects of Growth Regulators, Media and Explant Types on Microtuberization of Potato

Microtubers (MT) are an innovative approach for nuclear seed production in potato (Solanum tuberosum L.). They are produced under in vitro conditions using different protocols. However, content and type of growth media as well as explant types significantly affect the MT production efficiency. This study was conducted to develop an efficient protocol for MT production by evaluating different growth regulators at different concentrations, types of growth media and explants in two separate experiments. In Experiment 1, the effects of six different growth regulators, and their three application rates on MT formation were compared. The effects of two growth media and three explant types on MT production were compared in Experiment 2. As a result, our studies indicated that cotton based liquid MS medium containing 0.1 mg L−1 Thidiazuron, and using whole plants as explant can be used for efficient MT production in potato.