Three-dimensional structured illumination microscopy and its application to chromosome structure

被引：0
作者：
Peter M. Carlton
机构：
[1] University of California,Department of Biochemistry and Biophysics
[2] San Francisco,undefined
来源：
Chromosome Research | 2008年 / 16卷
关键词：
chromosome; imaging; meiosis; microscopy; structured illumination; synaptonemal complex;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
This review discusses the exploration of chromosome structure with a recently developed high-resolution microscopy technique, three-dimensional structured illumination microscopy (3dSIM). 3dSIM surpasses the diffraction limit of conventional widefield optical microscopy, increasing the level of detail in images by a factor of 2, while retaining the sample preparation methods, ease of use and flexibility of conventional microscopy. Special attention will be given to the ways in which imaging beyond the diffraction limit can shed light on the structural organization of meiotic chromosomes.
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页码：351 / 365
页数：14
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