A Thorough Dynamic Interpretation of Residual Dipolar Couplings in Ubiquitin

被引：0
作者：
Nils A. Lakomek
Teresa Carlomagno
Stefan Becker
Christian Griesinger
Jens Meiler
机构：
[1] Max Planck Institute for Biophysical Chemistry,NMR based structural biology
[2] Vanderbilt University,Center of Structural Biology, Department of Chemistry
来源：
Journal of Biomolecular NMR | 2006年 / 34卷
关键词：
dynamical averaging; model free approach; nuclear magnetic resonance (NMR); residual dipolar couplings (rdcs); ubiquitin;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
The presence of slow motions with large amplitudes, as detected by measurements based on residual dipolar couplings [Peti, W., Meiler, J., Brueschweiler, R. and Griesinger, C. (2002) J. Am. Chem. Soc., 124, 5822–5833], has stirred up much discussion in recent years. Based on ubiquitin NH residual dipolar couplings (rdcs) measured in 31 different alignment conditions, a model-free analysis of structure and dynamics [Meiler, J., Peti, W., Prompers, J., Griesinger, C. and Brueschweiler, R. (2001) J. Am. Chem. Soc., 123, 6098–6107] is presented. Starting from this broad experimental basis, rdc-based order parameters with so far unattained accuracy were determined. These rdc-based order parameters underpin the presence of new modes of motion slower than the inverse overall tumbling correlation time. Amplitudes and anisotropies of the motion were derived. The effect of structural noise on the results was proven to be negligible.
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页码：101 / 115
页数：14
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