Preferential Interaction of Chymotrypsinogen in Aqueous Solutions of Polyols at 298.15 K

Preferential interactions of α-chymotrypsinogen in water and aqueous solutions of ethylene glycol, glycerol, erythritol, sorbitol, and inositol were studied from precise density measurements under conditions of constant molality and constant chemical potential; to achieve the last condition, solutions were brought under equilibrium dialysis. The density measurements were performed at 298.15 K and they were used to determine the preferential interaction parameters. The results confirm a significant correlation between the numbers of hydroxyl groups of the polyols with the stability of α-chymotrypsinogen.