Hemolysate-induced Expression of Intercellular Adhesion Molecule-1 and Monocyte Chemoattractant Protein-1 Expression in Cultured Brain Microvascular Endothelial Cells via Through ROS-dependent NF-κB Pathways

被引：0
作者：
Hua Lu
Ji-Xin Shi
Dong-Mei Zhang
Jie Shen
Yi-Xing Lin
Chun-Hua Hang
Hong-Xia Yin
机构：
[1] Clinical School of Nanjing University,Department of Neurosurgery, Jinling Hospital
[2] Nanjing University,The State Key Laboratory of Pharmaceutical Biotechnology and Department of Biochemistry, College of Life Sciences
来源：
Cellular and Molecular Neurobiology | 2009年 / 29卷
关键词：
Brain microvascular endothelial cells; Hemolysate; Inflammation; Intercellular adhesion molecule-1; Monocyte chemoattractant protein-1; NF-κB; Reactive oxygen species;
D O I：
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中图分类号：
学科分类号：
摘要：
In order to determine the possible effects of hemolysate on brain microvascular endothelial cells (BMECs), we examined the effects of hemolysate on the expression of intercellular adhesion molecule-1 (ICAM-1) and monocyte chemoattractant protein-1 (MCP-1), generation of reactive oxygen species (ROS), and NF-κB activation in rat BMECs. Hemolysate induced the expression of ICAM-1 and MCP-1 in endothelial cells. In addition, hemolysate stimulated nuclear translocation of the p65 subunit of NF-κB, and NF-κB DNA-binding activity in BMECs. Furthermore, hemolysate increased ROS generation, and hemolysate-induced ICAM-1and MCP-1 expression and NF-κB activation were abrogated in the presence of the direct scavenger of ROS. Taken together, our results indicate that hemolysate can induce inflammatory responses that increase expression of ICAM-1 and MCP-1, through ROS-dependent NF-κB activation in BMECs.
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页码：87 / 95
页数：8
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