Review of micro/nano technologies and theories for electroporation of biological cells

被引：0
作者：
YiKuen Lee
PeiGang Deng
机构：
[1] Hong Kong University of Science and Technology,Department of Mechanical Engineering, Division of Biomedical Engineering, KAUST
[2] Wuhan Institute of Technology,HKUST Micro/Nanofluidics Laboratory
来源：
Science China Physics, Mechanics and Astronomy | 2012年 / 55卷
关键词：
electroporation; MEMS; microfluidics; DNA transfection; drug delivery; cell membrane; electropermeabilization; electrotransfection; electrointernalization;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Electroporation (EP) is one of the important techniques for the introduction of genes and drugs into cells with intense pulsed electric field to induce nanometer-sized electropores on cell membranes. Recently, micro/nano technology has been applied to many novel micro EP devices which can not only significantly increase uptake of biomolecules, DNA transfection and cell viability, but also enable large-scale single-cell EP. However, most EP theories developed in the past three decades can not precisely predict the experimental results of EP of biological cells. With the advanced micro EP chips for large-scale single-cell EP experiments, more precise EP theoretical models can be developed to describe the complicated multiscale dynamic behavior of EP.
引用
收藏
页码：996 / 1003
页数：7
相关论文
共 136 条
  • [1] Neumann E.(1972)Permeability changes induced by electric impulses in vesicular membranes J Membr Biol 10 279-290
  • [2] Rosenheck K.(1982)Gene transfer into mouse lyoma cells by electroporation in high electric fields EMBO J 1 841-845
  • [3] Neumann E.(2003)Electroporation: Theory and methods, perspectives for drug delivery, gene therapy and research Acta Phys Scand 177 437-447
  • [4] Schaefer-Ridder M.(2002)The development of electroporation Science 295 277-281
  • [5] Wang Y.(2000)Medical applications of electroporation Plasma Sci IEEE Trans 28 206-223
  • [6] Gehl J.(2000)Electroporation: Bio-electro-chemical mass transfer at the nano scale Microscale Thermophys Eng 4 147-159
  • [7] Heller R.(1997)Shock jocks Scientist 11 19-21
  • [8] Dev S. B.(1982)Electric field-mediated fusion and related electrical phenomena Biochim Biophys Acta 694 227-277
  • [9] Rabussay D. P.(1996)Theory of electroporation: A review Bioelectrochem Bioenerg 41 135-160
  • [10] Widera G.(1997)Sensitivity of transmembrane voltage induced by applied electric fields—a theoretical analysis Bioelectrochem Bioenerg 43 285-291
12345678910