Oxidative stress occurs when there is an imbalance between the production of free radicals and the detoxification of these reactive products. Regular exercise greatly increases the production of free radicals in humans, while excessive exercise could lead to cellular and tissue damage. This paper presents a review of studies focusing on exercise-induced oxidative stress, sufficient intensity of aerobic or anaerobic exercises, and how exercise volume and duration could increase free radicals in humans. Furthermore, this review examines the effects of chronic or acute vitamin C supplementation in minimising oxidative stress during exercise. In this regard, vitamin C is commonly consumed as an antioxidant supplement by endurance athletes to minimise exercise-induced oxidative stress, augment recovery and improve performance. However, there is still a lack of evidence regarding the antioxidant effects of vitamin C supplements during exercise. Based on the review, it could be concluded healthy, physically active individuals might not require regular intakes of non-physiological dosages of vitamin C. On the other hand, studies found that regular exercise could lead to higher antioxidant production that could be beneficial in mitigating oxidative stress and improving individuals’ health.