Molecular cloning and transcriptional regulation of two γ-carbonic anhydrase genes in the green macroalga Ulva prolifera

被引:0
作者
Yu Wang
Feng Liu
Manman Liu
Shitao Shi
Yuping Bi
Nansheng Chen
机构
[1] Chinese Academy of Sciences,CAS Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology
[2] Pilot National Laboratory for Marine Science and Technology (Qingdao),Marine Ecology and Environmental Science Laboratory
[3] Chinese Academy of Sciences,Center for Ocean Mega
[4] Shandong Academy of Agricultural Sciences,Science
来源
Genetica | 2021年 / 149卷
关键词
Carbonic anhydrase; Environmental factor; Green tide; Transcriptional regulation;
D O I
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中图分类号
学科分类号
摘要
Ulva prolifera O.F. Müller (Ulvophyceae, Chlorophyta) is well known as a typical green-tide forming macroalga which has caused the world’s largest macroalgal blooms in the Yellow Sea of China. In this study, two full-length γ-carbonic anhydrase (γ-CA) genes (UpγCA1 and UpγCA2) were cloned from U. prolifera. UpγCA1 has three conserved histidine residues, which act as an active site for binding a zinc metal ion. In UpγCA2, two of the three histidine residues were replaced by serine and arginine, respectively. The two γ-CA genes are clustered together with other γ-CAs in Chlorophyta with strong support value (100% bootstrap) in maximum likelihood (ML) phylogenetic tree. Quantitative real-time PCR (qRT-PCR) analysis showed that stressful environmental conditions markedly inhibited transcription levels of these two γ-CA genes. Low pH value (pH 7.5) significantly increased transcription level of UpγCA2 not UpγCA1 at 12 h, whereas high pH value (pH 8.5) significantly inhibited the transcription of these two γ-CA genes at 6 h. These findings enhanced our understanding on transcriptional regulation of γ-CA genes in response to environmental factors in U. prolifera.
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页码:63 / 72
页数:9
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