Identification of a Gene Involved in the Control of the Root System Development in Arabidopsis thaliana

被引:0
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作者
A. A. Tomilov
N. B. Tomilova
O. A. Ogarkova
V. A. Tarasov
机构
[1] Vavilov Institute of General Genetics,
[2] Russian Academy of Sciences,undefined
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关键词
Nucleotide; Nucleotide Sequence; Root System; Arabidopsis Thaliana; System Development;
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摘要
Genetic and molecular genetic analysis of a lethal root mutant of Arabidopsis thaliana was carried out. The mutant was obtained from a collection created earlier by means of insertion mutagenesis. The mutation was found to be recessive. It was caused by an insertion of the T region of vector pLD3 used for transformation of germinating seeds when creating the collection of insertion mutants. A 118-bp DNA fragment flanking the left border of the insertion was isolated using the TAIL PCR technique, and its nucleotide sequence was determined. Computer analysis of this DNA region demonstrated that it was located in exon 32 of the YUP8H12R.44 gene in chromosome 1.
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页码:30 / 38
页数:8
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