Comparison of Mycobacterium tuberculosis drug susceptibility using solid and liquid culture in Nigeria

被引：20

作者：

Lawson L. ^{[1
]}

Emenyonu N. ^{[1
]}

Abdurrahman S.T. ^{[2
]}

Lawson J.O. ^{[1
]}

Uzoewulu G.N. ^{[3
]}

Sogaolu O.M. ^{[4
]}

Ebisike J.N. ^{[5
]}

Parry C.M. ^{[6
]}

Yassin M.A. ^{[7
]}

Cuevas L.E. ^{[7
]}

机构：

[1] Zankli Medical Centre, Abuja, Shehu YarAdua Way

[2] National Tuberculosis and Leprosy Control Programme, Abuja

[3] Nnamdi Azikiwe Teaching Hospital, Nnewi

[4] University College Hospital (UCH), Ibadan

[5] Wuse General Hospital, Abuja

[6] Mahidol-Oxford Tropical Medicine Research Unit, Angkor Hospital for Children, Siem Reap

[7] School of Tropical Medicine, Liverpool

来源：

BMC Research Notes | / 6卷 / 1期

关键词：

Drug sensitivity; First-line anti-TB drugs; Liquid culture; Multi-drug resistant tuberculosis; Solid culture;

D O I：

10.1186/1756-0500-6-215

中图分类号：

学科分类号：

摘要：

Background: This study compares Mycobacterium tuberculosis culture isolation and drug sensitivity testing (DST) using solid (LJ) and liquid (BACTEC-MGIT-960) media in Nigeria. Methods. This was a cross sectional survey of adults attending reference centres in Abuja, Ibadan and Nnewi with a new diagnosis of pulmonary tuberculosis (TB) or having failed the first-line TB treatment. Patients were requested to provide three sputum specimens for smear-microscopy and culture on LJ and BACTEC-MGIT-960. Positive cultures underwent DST for streptomycin, isoniazid, rifampicin and ethambutol. Results: 527 specimens were cultured. 428 (81%) were positive with BACTEC-MGIT-960, 59 (11%) negative, 36 (7%) contaminated and 4 (1%) had non-tuberculosis mycobacteria (NTM). 411 (78%) LJ cultures were positive, 89 (17%) negative, 22 (4%) contaminated and 5 (1%) had NTM. The mean (SD) detection time was 11 (6) and 30 (11) days for BACTEC-MGIT-960 and LJ. DST patterns were compared in the 389 concordant positive BACTEC-MGIT-960 and LJ cultures. Rifampicin and isoniazid DST patterns were similar. Streptomycin resistance was detected more frequently with LJ than BACTEC-MGIT-960 and ethambutol resistance was detected more frequently with BACTEC-MGIT-960 than LJ, but differences were not statistically significant. MDR-TB was detected in 27 cases by LJ and 25 by BACTEC-MGIT-960 and using both methods detected 29 cases. Conclusions: There was a substantial degree of agreement between the two methods. However using the two in tandem increased the number of culture-positive patients and those with MDR-TB. The choice of culture method should depend on local availability, cost and test performance characteristics. © 2013 Lawson et al.; licensee BioMed Central Ltd.

引用

共 17 条

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