Shoot regeneration from leaf explants of Brunfelsia calycina

被引：0
|
作者：
Raya Liberman
Liat Shahar
Ada Nissim-Levi
Dalia Evenor
Moshe Reuveni
Michal Oren-Shamir
机构：
[1] ARO Volcani Center,Department of Ornamental Horticulture
来源：
Plant Cell, Tissue and Organ Culture (PCTOC) | 2010年 / 100卷
关键词：
Regeneration; Solanaceae;
D O I：
暂无
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学科分类号：
摘要：
A protocol for plantlet regeneration through shoot formation was developed for the neotropical shrub Brunfelsia calycina. This shrub is unique in its change in flower color from dark purple to white. Explants from young and mature leaves were incubated on MS medium (pH 5.7, 30 g/l sucrose, 7.5 g/l agar) with various combinations of Indole-3-acetic acid (IAA) and 6-Benzyladenine (BA) under a 16 h photoperiod at a constant temperature of 25°C. Shoot emergence was best at 4.44 μM BA and 2.85 μM IAA for young leaf explants, and at 8.88 μM BA, 2.85 μM IAA for mature leaf explants. When shoots were transferred to MS medium supplemented with 1.23–2.46 μM indole butrytic acid (IBA), they developed roots.
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页码：345 / 348
页数：3
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