Genetic diversity of Fusarium oxysporum f. sp. lentis populations causing wilt of lentil in India

被引：0

作者：

Hiremani N.S. ^{[1
,2
]}

Dubey S.C. ^{[1
,3
]}

机构：

[1] Division of Plant Pathology, ICAR-Indian Agricultural Research Institute, New Delhi

[2] Division of Crop Protection, ICAR-CICR, Nagpur

[3] Division of Plant Quarantine, ICAR-NBPGR, New Delhi

来源：

Indian Phytopathology | 2019年 / 72卷 / 4期

关键词：

Fusarium oxysporum f. sp. Lentis; Genetic diversity; Lentil wilt; RAPD; SSR;

D O I：

10.1007/s42360-019-00126-9

中图分类号：

学科分类号：

摘要：

Fusarium oxysporum is associated with vascular wilt disease in most of the crops. Identification of Fusarium spp. by morphological characters are highly variable, thus DNA-based techniques such as RAPD and SSR markers are widely used in the detection and genetic diversity analysis. In this study, fifty F. oxysporum f. sp. lentis (Fol) isolates from 7 different lentil cultivating Indian states were analyzed for the genetic diversity by using 14 RAPD and 9 SSR primers. Results revealed the existence of high genetic variability among the isolates. A total of 125 reproducible bands were amplified by RAPD primers, out of which 121 were polymorphic (96.8%) and only four were monomorphic (3.2%). Primers OPA 12, OPC 14, OPF 8, OPM 12 and OPY 20 showed the highest polymorphism. UPGMA analysis differentiated the isolates into six clusters at 25% similarity coefficient. Nine SSR primers produced 59 bands with 96.6% polymorphism. The primers MB 2, MB 9 and MB 11 were highly informative and showed maximum polymorphism (100%). UPGMA analysis at 30% genetic similarity grouped the isolates into six clusters. It was evident that the isolates belonging to different locations were grouped into different clusters representing different races. However, the analysis partially corresponded to the races of Fol and the lentil growing regions of India. Nevertheless, this information is highly useful for the breeders to plan the breeding strategies to develop resistant cultivars against Fol looking into existence of races in a particular region and considering the diversity among them. © 2019, Indian Phytopathological Society.

引用

页码：657 / 663

页数：6

共 21 条

[1]

Agrawal S.C., Singh K., Lal S.S., Plant protection of lentil in India, Lentil in South Asia ICAR-ICARDA Seminar, pp. 147-167, (1991)

[2]

Al-Husien N.H., Hamwieh A., Ahmed S., Bayaa B., Genetic diversity of Fusarium oxysporum f. sp. lentis population affecting lentil in Syria, J Phytopathol, 165, pp. 306-312, (2017)

[3]

Baayen R.P., van Dreven F., Krijger M.C., Waalwijk C., Genetic diversity in Fusarium oxysporum f. sp. dianthi and Fusarium redolens f. sp. dianthi, Eur J Plant Pathol, 103, pp. 395-408, (1997)

[4]

Bayraktar H., Genetic diversity and population structure of Fusarium oxysporum f. sp. cepae, the causal agent of Fusarium basal plate rot on onion, using RAPD markers, J Agric Sci, 16, pp. 139-149, (2010)

[5]

Belabid L., Baum M., Fortas Z., Bouznad Z., Eujayl I., Pathogenic and genetic characterisation of Algerian isolates of Fusarium oxysporum f. sp. lentis by RAPD and AFLP analysis, Afr J Biotechnol, 3, pp. 25-31, (2004)

[6]

Bogale M., Wingfield B.D., Wingfield M.J., Steenkamp E.T., Simple sequence repeat markers for species in the Fusarium oxysporum complex, Mol Ecol Note, 5, pp. 622-624, (2005)

[7]

Bogale M., Wingfield B.D., Wingfield M.J., Steenkamp E.T., Characterisation of Fusarium oxysporum isolates from Ethiopia using AFLP, SSR and DNA sequence analyses, Fungal Divers, 23, pp. 51-66, (2006)

[8]

Castano R., Scherm B., Aviles M., Genetic Diversity of Fusarium oxysporum f. sp. dianthi in Southern Spain, J Mycol, (2014)

[9]

Datta J., Lal N., Genetic diversity of Fusarium wilt races of pigeon pea in major regions of India, African Crop, 21, pp. 201-211, (2013)

[10]

Datta S., Choudhary R.G., Shamim M.D., Singh R.K., Dhar V., Molecular diversity in Indian isolates of Fusarium oxysporum f. sp. lentis inciting wilt disease in lentil (Lens culinaris Medik), Afr J Biotechnol, 10, pp. 7314-7323, (2011)

← 1 2 3 →