Oxidative-protective effects of Tinospora cordifolia extract on plasma and spleen cells after experimental ochratoxicosis

被引：10

作者：

Karamalakova Y. ^{[1
]}

Nikolova G. ^{[1
]}

Adhikari M. ^{[2
]}

Stoev S. ^{[3
]}

Agarwal P. ^{[2
]}

Gadjeva V. ^{[1
]}

Zhelev Z. ^{[1
]}

机构：

[1] Department Chemistry and Biochemistry, Medical Faculty, Trakia University, 11 Armeiska Str, Stara Zagora

[2] Radiation Biotechnology Group, Institute of Nuclear Medicine and Allied Sciences, Delhi

[3] Faculty of Veterinary Medicine, Department of General and Clinical Pathology, Trakia University, Students campus, Stara Zagora

来源：

Comparative Clinical Pathology | 2018年 / 27卷 / 6期

关键词：

8-OHdG; Ochratoxicosis; ROS/RNS; Tinospora cordifolia;

D O I：

10.1007/s00580-018-2761-y

中图分类号：

学科分类号：

摘要：

The importance of Tinospora cordifolia extract was investigated for its possible protective effect in spleen and blood in male ICR albino mice against ochratoxin A-induced toxicity (OTA). OTA instigates oxidative changes and results in the overproduction of free radicals, changes in body/organ weight, and the food consumption and is considered as a risk factor for animals and humans. Reactive oxygen species (ROS), reactive nitrogen species (RNS), and consequent lipid-peroxidative damages caused by OTA are considered to be the main mechanisms leading to oxidative stress disorders in organs and blood. TC oral administrations prevent the physiological status of animals and improve the biochemical parameters of the urine and plasma. Moreover, EPR analysis show that TC administration decreased Asc• and NO• radicals and ROS productions in the spleen and serum, even in OTA-treated group. The significant decrease in MDA formation in the spleen and serum and the increased expression of SOD activity in TC and TC + OTA groups confirm the positive modulatory effect of TC extract on the cellular antioxidant system. TC and TC + OTA treatment caused significant reduction of genotoxic potential (8-OHdG) of OTA, followed by a decreased oxidative activity and gradual recovery of ROS-induced DNA damage. The results suggested that TC extract protect against OTA-induced oxidative disorders and other abnormalities. As typical antioxidant TC could protect splenic macrophages and regain intracellular antioxidant capacity. © 2018, Springer-Verlag London Ltd., part of Springer Nature.

引用

页码：1487 / 1495

页数：8

共 55 条

[1]

Agarwal A., Malini S., Bairy K.L., Rao M.S., Effect of Tinospora cordifolia on learning and memory in normal and memory deficit rats, Indian J Pharm, 34, 5, pp. 339-349, (2002)

[2]

Birben E., Sahiner U.M., Sackesen C., Erzurum S., Kalayci O., Oxidative stress and antioxidant defense, World Allergy Organ J, 5, 1, pp. 9-19, (2012)

[3]

Buettner G.R., Jurkiewicz B.A., Ascorbate free-radical as a marker of oxidative stress––an EPR study, Free Radic Biol Med, 14, 1, pp. 49-55, (1993)

[4]

Cavin C., Delatour T., Marin-Kuan M., Holzhauser D., Higgins L., Bezencon C., Guignard G., Junod S., Richoz-Payot J., Gremaud E., Hayes J.D., Nestler S., Mantle P., Schilter B., Reduction in antioxidant defenses may contribute to ochratoxin a toxicity and carcinogenicity, Toxicol Sci, 96, pp. 30-39, (2007)

[5]

Chakraborty B., Sengupta M., Supporting the immune system through functional modulation of carbon tetrachloride intoxicated splenic macrophages by administering Tinospora cordifolia, J Appl Pharm Sci, 2, 7, pp. 117-124, (2012)

[6]

Creppy E.E., Kane A., Dirheimer G., Lafarge-Frayssinet C., Mousset S., Frayssinet C., Genotoxicity of ochratoxin A in mice: DNA single-strand break evaluation in spleen, liver and kidney, Toxicol Lett, 28, 1, pp. 29-35, (1985)

[7]

Denli M., Perez J.F., Ochratoxins in feed, a risk for animal and human health: control strategies, Toxins, 2, 5, pp. 1065-1077, (2010)

[8]

Elaroussi M.A., Mohamed F.R., El Barkouky E.M., Et al., Experimental ochratoxicosis in broiler chickens, Avian Pathol, 35, 4, pp. 263-269, (2006)

[9]

Gacche R.N., Dhole N.A., Profile of aldose reductase inhibition, anti-cataract and free radical scavenging activity of selected medicinal plants: an attempt to standardize the botanicals for amelioration of diabetes complications, Food Chem Toxicol, 49, 8, pp. 1806-1813, (2011)

[10]

Gupta R., Sharma V., Ameliorative effects of Tinospora cordifolia root extract on histopathological and biochemical changes induced by aflatoxin-b (1) in mice kidney, Toxicol Int, 18, 2, pp. 94-98, (2011)

← 1 2 3 4 5 6 →