Characterization of the putative α subunit of a heterotrimeric G protein in rice

被引：3
作者：
Yukimoto Iwasaki
Teruhisa Kato
Toshio Kaidoh
Atsushi Ishikawa
Tadashi Asahi
机构：
[1] Fukui Prefectual University,Department of Bioscience, Faculty of Biotechnology
来源：
Plant Molecular Biology | 1997年 / 34卷
关键词：
ADP ribosylation; α subunit; cholera toxin; heterotrimeric G protein; rice; signal transduction;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
A recombinant protein with a cDNA that encodes the putative α subunit of a rice heterotrimeric G protein was synthesized in Escherichia coli and purified. The recombinant protein (rGrice α) with an apparent molecular mass of 45 kDa was bound with guanosine 5′-(3-O-thio)triphosphate with an apparent association constant (kapp) of 0.36. The protein also hydrolyzed GTP and its Kcat was 0.44. rGrice α was ADP-ribosylated by activated cholera toxin.
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页码：563 / 572
页数：9
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