Imbalance between production and scavenging of hydroxyl radicals in patients maintained on hemodialysis

被引：7

作者：

Shirai S. ^{[1
]}

Ominato M. ^{[2
]}

Shimazu T. ^{[3
]}

Toyama K. ^{[1
]}

Ogimoto G. ^{[1
]}

Fujino T. ^{[1
]}

Yasuda T. ^{[1
]}

Sato T. ^{[1
]}

Maeba T. ^{[1
]}

Owada S. ^{[2
]}

Kimura K. ^{[1
]}

机构：

[1] Division of Nephrology and Hypertension, Department of Internal Medicine, St. Marianna University School of Medicine, Miyamae-ku, Kawasaki 216-8511

[2] Akabane-Chuo Hospital, Tokyo

[3] Tachibana-dai Hospital, Yokohama

来源：

Clinical and Experimental Nephrology | 2005年 / 9卷 / 4期

关键词：

Electron spin resonance; Endstage renal failure; Hemodialysis; Hydroxyl radicals; Oxidative stress;

D O I：

10.1007/s10157-005-0374-7

中图分类号：

学科分类号：

摘要：

Background. Reactive oxygen species are as being related to the pathophysiology of endstage renal disease (ESRD). We measured the plasma hydroxyl radical (·OH)-producing ability and ·OH-scavenging activity in patients with ESRD to clarify the pathophysiological states involved. Methods. We used electron spin resonance to measure plasma N-t-butyl-α-phenylnitron radical spin adduct (pPBN rsa) as ·OH-producing ability and plasma 3,3,5,5-tetramethyl-1-pyrroline-N-oxide radical spin adduct (pM4PO rsa) as ·OH-scavenging activity. Oxidative injuries were evaluated by determining oxidised low-density lipoprotein (Ox-LDL). Results. The pPBN rsa of the ESRD patients was lower than that of the controls (1.83 vs 2.94μmol/g protein). The pM4PO rsa of the ESRD patients was higher than that of the controls (3.85 vs 3.15mmol l-ascorbic acid 2-[3,4-dihydro-2,5,7,8-tetramethyl-2-(4,8,12-trimethyltridecyl) -2H-1-benzopyran-6-yl hydrogen phosphate] potassium salt (EPC-K1)/g protein). The pPBN rsa and pM4PO rsa were correlated, both in the ESRD patients and in the controls (r = 0.47 and r = 0.53). Ox-LDL was correlated with hemodialysis (HD) duration (r = 0.49) and was negatively correlated with pPBN rsa (r = -0.54), which indicates that oxidative stress was increased as HD therapy was prolonged and suppressed pPBN rsa. Conclusions. There was an imbalance between ·OH-producing ability and ·OH-scavenging activity, in the ESRD patients, and this may be responsible for compromising the health of ESRD patients. © Japanese Society of Nephrology 2005.

引用

页码：310 / 314

页数：4

共 19 条

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