Counting colonies of clonogenic assays by using densitometric software

被引：0
|
作者：
Maximilian Niyazi
Ismat Niyazi
Claus Belka
机构：
[1] CCC Tübingen,Department of Radiation Oncology
[2] Bureau for Technique and Documentation,undefined
来源：
Radiation Oncology | / 2卷
关键词：
Gray Level; Routine Activity; Interindividual Difference; Clonogenic Assay; Microscopical Evaluation;
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摘要：
Clonogenic assays are a useful tool to test whether a given cancer therapy can reduce the clonogenic survival of tumour cells. A colony is defined as a cluster of at least 50 cells which can often only be determined microscopically. The process of counting colonies is very extensive work and so we developed software that is able to count the colonies automatically from scanned flasks. This software is made freely available by us with a detailed description how to use and install the necessary features.
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