Biochemical analysis of the interaction between Arabidopsis thaliana AtMYB30 transcription factor and its DNA specific target site

AtMYB30 is well known as a transcription factor involved in cell death processes during the hypersensitive response against biotic stress in Arabidopsis thaliana. Despite the relevant function played by R2R3-MYB transcription factors in the regulation of plant gene expression, limited information exists about how these proteins interact with their DNA targets, their specificity, and binding affinity. Our findings confirm the binding of a specific DNA sequence (5′-AAACCAA) to AtMYB30, binding affinities were calculated, and the effect of DNA on AtMYB30 secondary structure was evaluated. Our data shed new light on the interaction between DNA and this important member of a plant-specific transcriptional regulator family.