Resistance of apple leaves to infection by Colletotrichum fructicola acts independently of hypersensitive reaction and PR-1 and PR-10 gene expression

被引:0
作者
Mathias F. Rockenbach
Aline C. Velho
Sandra M. Alaniz
Marciel J. Stadnik
机构
[1] Federal University of Santa Catarina,Laboratory of Plant Pathology, Agricultural Science Center
[2] University of the Republic,Department of Plant Protection, Faculty of Agronomy
来源
Tropical Plant Pathology | 2018年 / 43卷
关键词
Glomerella leaf spot; Hypersensitive response; Pathogenesis-related genes;
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中图分类号
学科分类号
摘要
Colletotrichum fructicola is currently one of the most important fungal pathogens affecting apple production in the subtropics. This work was aimed at studying the resistance mechanisms of apple leaves during early stages of pathogen infection. Seedlings from ‘Fuji’ × ‘Gala’ crosses, resulting in resistant and susceptible plants (1:1) were used in an assay model. Disease severity reached 19% on susceptible plants at 7 days after inoculation with C. fructicola. Enzymatic activity of ascorbate peroxidase, guaiacol peroxidase, and superoxide dismutase were similar between resistant and susceptible leaves, whereas glutathione reductase activity was enhanced in inoculated resistant plants. Hydrogen peroxide (H2O2) leaf content showed a decrease only in inoculated resistant leaves at 12 h after inoculation (hai). H2O2 accumulation associated with the hypersensitive reaction (HR) was observed at a low frequency (ca. one reaction in 3000 penetration attempts) in resistant leaves. Pathogenesis-related 1 (PR-1) gene expression was not affected by inoculation with C. fructicola up to 48 hai. Whereas PR-10 gene expression was significantly increased in leaves of both genotypes at 24 and 48 hai. Results suggest that the monogenic recessive resistance of apple to GLS acts independently of HR and both PR-1 and PR-10 genes expression.
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页码:360 / 370
页数:10
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共 211 条
[1]  
Acevedo-Garcia J(2014)Magical mystery tour: MLO proteins in plant immunity and beyond New Phytologist 204 273-281
[2]  
Kusch S(2013)Cultivar-specific and ulvan-induced resistance of apple plants to Glomerella leaf spot are associated with enhanced activity of peroxidases Acta Scientarum 35 287-293
[3]  
Panstruga R(2008)Fosfito de potássio e ulvana no controle da mancha foliar da gala em macieira Tropical Plant Pathology 33 148-152
[4]  
Araújo L(2014)Ulvan effect on conidial germination and appressoria formation of Phytoparasitica 42 631-613
[5]  
Stadnik MJ(2009)A locus conferring resistance toColletotrichum higginsianumis shared by four geographically distinct Arabidopsis accessions The Plant Journal 60 602-170
[6]  
Araújo L(2006)PR genes of apple: identification and expression in response to elicitors and inoculation with BMC Plant Biology 6 23-1640
[7]  
Stadnik MJ(2006)Ontogenetic variation in chemical and physical characteristics of adaxial apple leaf surfaces Phytochemistry 67 161-352
[8]  
Borsato LC(2006)Epicatechin and catechin may prevent coffee berry disease by inhibition of appressorial melanization of Biotechnology Letters 28 1637-356
[9]  
Valdebenito-Sanhueza RM(2009)Cell wall appositions: the first line of defence Journal of Experimental Botany 60 351-323
[10]  
Araújo L(2005)Antimicrobial activity of flavonoids International Journal of Antimicrobial Agents 26 343-281