The In Vivo Synthesis and Accumulation of Lectin in Developing Seeds of Black Gram (Vigna mungo L. Hepper)

被引：0
作者：
K.N. SUSEELAN
R. MITRA
C.R. BHATIA
T. GOPALAKRISHNA
机构：
[1] Bhabha Atomic Research Centre,Nuclear Agriculture and Biotechnology Division
[2] 6,Nuclear Agriculture and Biotechnology Division
[3] Madhulika,undefined
[4] 17,undefined
[5] Rohini,undefined
[6] Bhabha Atomic Research Centre,undefined
来源：
Plant Foods for Human Nutrition | 2004年 / 59卷
关键词：
Biosynthesis; Black gram; Lectin; Legume; Radial immunodiffusion;
D O I：
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中图分类号：
学科分类号：
摘要：
Black gram (Vigna mungo L. Hepper) seed contains two D-galactose-specific lectin species, BGL-I and BGL-II, identified on the basis of elution from ion exchange column and immunochemical cross-reactivity. BGL-I consisted of two monomeric lectins, BGL-I-1 and BGL-I-2, of relative molecular weights 94 and 89 kDa, respectively. BGL-II is another monomeric lectin with a molecular weight of 83 kDa. The in vivo synthesis studies using pulse-chase experiment showed that BGL-II lectin was synthesized as early as 14 days after flowering (DAF). The 94-kDa BGL-I-1 lectin was synthesized around 17 DAF. There was no cotranslational or posttranslational modification of the lectin proteins. The amount of lectin in developing seeds was determined by radial immunodiffusion assay technique. The maximum amount of lectin per seed was found at 28 DAF.
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页码：123 / 128
页数：5
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