Antioxidant and phytochemical analysis of Ranunculus arvensis L. extracts

被引：77

作者：

Bhatti M.Z. ^{[1
]}

Ali A. ^{[2
]}

Ahmad A. ^{[3
]}

Saeed A. ^{[4
]}

Malik S.A. ^{[1
]}

机构：

[1] Department of Biochemistry, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad

[2] Institute of Biomedical Sciences, School of Life Science, East China Normal University, 500 Dongchuan Road, Shanghai

[3] Department of Biotechnology, Abdul Wali Khan University, Mardan

[4] Department of Biological Sciences, Gomal University, Dera Ismail Khan

来源：

BMC Research Notes | / 8卷 / 1期

关键词：

Antioxidant; Flavonoid content; HPLC; Phenolic content; Ranunculus arvensis;

D O I：

10.1186/s13104-015-1228-3

中图分类号：

学科分类号：

摘要：

Background: Ranunculus arvensis L. (R. arvensis) has long been used to treat a variety of medical conditions such as arthritis, asthma, hay fever, rheumatism, psoriasis, gut diseases and rheumatic pain. Here, we screened R. arvensis for antioxidant activity, phytochemical and high performance liquid chromatography (HPLC) analyses. Methods: The chloroform, chloroform:methanol, methanol, methanol:acetone, acetone, methanol:water and water extracts of R. arvensis were examined for DPPH (1, 1-diphenyl-2-picrylhydrazyl) free radical scavenging assay, hydrogen peroxide scavenging assay, phosphomolybdenum assay, reducing power assay, flavonoid content, phenolic content and high performance liquid chromatography analysis. Results: Significant antioxidant activity was displayed by methanol extract (IC 50 34.71 ± 0.02) in DPPH free radical scavenging assay. Total flavonoids and phenolics ranged 0.96-6.0 mg/g of extract calculated as rutin equivalent and 0.48-1.43 mg/g of extract calculated as gallic acid equivalent respectively. Significant value of rutin and caffeic acid was observed via high performance liquid chromatography. Conclusions: These results showed that extracts of R. arvensis exhibited significant antioxidant activities. Moreover, R. arvensis is a rich source of rutin, flavonoids and phenolics. © 2015 Bhatti et al.

引用

共 33 条

[21]

Velioglu Y.S., Mazza G., Gao L., Oomah B.D., Antioxidant activity and total phenolics in selected fruits, vegetables, and grain products, J Agri Food Chem, 46, pp. 4113-4117, (1998)

[22]

Zu Y., Li C., Fu Y., Zhao C., Simultaneous determination of catechin, rutin, quercetin kaempferol and isorhamnetin in the extract of sea buckthorn (Hippophae rhamnoides L.) leaves by RP-HPLC with DAD, J Pharmaceut Biomed Anal, 41, pp. 714-719, (2006)

[23]

Kirca A., Arslan E., Antioxidant capacity and total phenolic content of selected plants from Turkey, Intern J Food Sci Technol, 43, pp. 2038-2046, (2008)

[24]

Meziti A., Meziti H., Boudiaf K., Mustapha B., Bouriche H., Polyphenolic profile and antioxidant activities of Nigella Sativa seed extracts in vitro and in vivo, World Acad Sci Eng Technol, 64, pp. 24-32, (2012)

[25]

Zengin G., Aktumsek A., Guler G.O., Cakmak Y.S., Yildiztugay E., Antioxidant properties of methanolic extract and fatty acid composition of Centaurea urvillei DC. Hayekiana Wagenitz, J Nat Prod, 5, pp. 123-132, (2011)

[26]

Shah K.A., Patel M.D., Parmar P.K., Patel R.J., In-vitro evaluation of antioxidant activity of Gymnema Sylvestre, Deccan J Nat Prod, 1, pp. 1-7, (2010)

[27]

Hazra B., Biswas S., Mandal N., Antioxidant and free radical scavenging activity of Spondias pinnata, BMC Complement Altern Med, 8, pp. 1-10, (2008)

[28]

Hachelaf A., Zellagui A., Touil A., Rhouati S., Chemical composition and analysis antifungal properties of Ranunculus arvensis, L. Pharmacophore, 4, 3, pp. 89-91, (2013)

[29]

Hussain I., Ullah R., Ullah R., Khurram M., Ullah N., Baseer A., Et al., Phytochemical analysis of selected medicinal plants, Afr J Biotechnol, 10, pp. 7487-7492, (2011)

[30]

Kaya G.I., Somer N.U., Konyalioglu S., Yalcin H.T., Yavasoglu N.U.K., Sarikaya B., Et al., Antioxidant and antibacterial activities of Ranunculus marginatus var. trachycarpus and R. sprunerianus. Turk, J Biol, 34, pp. 139-146, (2010)

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