Formation of liposome by microfluidic flow focusing and its application in gene delivery

被引：0
作者：
Rinbok Wi
Yeonsu Oh
Chanhee Chae
Do Hyun Kim
机构：
[1] Korea Advanced Institute of Science and Technology,Department of Chemical and Biomolecular Engineering
[2] Seoul National University,Department of Veterinary Pathology, College of Veterinary Medicine
来源：
Korea-Australia Rheology Journal | 2012年 / 24卷
关键词：
liposome; microfluidics; hydrodynamic flow focusing; gene delivery;
D O I：
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中图分类号：
学科分类号：
摘要：
We report the formation of liposomes in a simple procedure using a microfluidic hydrodynamic flow focusing method for the application in gene delivery. We fabricated microfluidic device using soft lithography and polydimethylsiloxane (PDMS) molding technique. Lipid-containing stream was surrounded by aqueous stream and liposomes were formed at the lipid-water interface. Size distribution of liposomes and zeta potential of liposome dispersion were investigated under various flow rate ratio (FRR) and processing temperature. Size distributions of liposomes were measured by dynamic light scattering (DLS), and zeta potential was measured to quantify the colloidal stability. Prepared liposomes were used as a vehicle for gene delivery, and the successful expression of delivered gene was observed by fluorescent microscope.
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页码：129 / 135
页数：6
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共 49 条
  • [1] Allen T.M.(1989)Liposomes with prolonged circulation times: factors affecting uptake by reticuloendothelial and other tissues Biochim. Biophys. Acta. 981 27-35
  • [2] Hansen C.(2003)Membrane Phospholipid Composition of Biochemistry (Moscow) 68 752-759
  • [3] Rutledge J.(1965) Affects Secretion of Periplasmic Alkaline Phosphatase into the Medium J. Mol. Biol. 13 238-252
  • [4] Badyakina A.O.(2011)Diffusion of univalent ions across the lamellae of swollen phospholipids J. Colloid Interface Sci. 357 243-251
  • [5] Koryakina Y. A.(1991)Continuous-flow production of polymeric micelles in microreactors: experimental and computational analysis Chem. Phys. Lipids 60 201-208
  • [6] Suzina N.E.(2005)Effects of solute concentration on the entrapment of solutes in phospholipid vesicles prepared by freeze-thaw extrusion Molecular Biology 39 260-268
  • [7] Nesmeyanova M.A.(2010)Unbalanced phospholipid composition of ACS Nano 4 2077-2087
  • [8] Bangham A.D.(1981) membranes affects P Biochim. Biophys. Acta. 677 453-461
  • [9] Standish M.M.(1984) promoter activity Nature Biotechnology 2 979-984
  • [10] Watkins J.C.(1994)Microfluidic mixing and the formation of nanoscale lipid vesicles Biochim. Biophys. Acta. 1190 99-107
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