A Quantitative Assay of Telomerase Activity

被引：0
作者：
Yuebo Gan
Jie Lu
Andy Johnson
M. Guillaume Wientjes
David E. Schuller
Jessie L-S Au
机构：
[1] The Ohio State University,College of Pharmacy
[2] Arthur James Cancer Hospital and Richard Solove Research Institute,Department of Otolaryngology
[3] The Ohio State University,undefined
[4] The Ohio State University,undefined
来源：
Pharmaceutical Research | 2001年 / 18卷
关键词：
telomerase; telomerase assay; TRAP; inhibitor;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Purpose. Telomerase is a ribonucleoprotein that extends telomeres at the ends of chromosome. Increased telomerase activity is associated with cellular immortality. The currently available assay for telomerase, i.e., telomeric repeat amplification protocol (TRAP), consists of 2 steps: (a) telomerase-mediated extension of an oligonucleotide primer by the enzyme-containing extracts of cells and tissues, and (b) amplification of the telomerase-extended primer products by polymerase chain reaction (PCR) and detection of the PCR products. It is generally accepted that the current TRAP assay lacks quantitative precision. The present study was to develop a quantitative telomerase assay with greater precision and sensitivity.
引用
收藏
页码：488 / 493
页数：5
相关论文
共 80 条
[1]  
Urquidi V.(2000)Role of telomerase in cell senescence and oncogenesis Ann. Rev. Med. 51 65-79
[2]  
Tarin D.(1999)Antiapoptosis role of telomerase in pheochromocytoma cells J. Biol. Chem. 274 7264-7271
[3]  
Goodison S.(1998)Telomere maintenance is dependent on activities required for end repair of double-strand breaks Curr. Biol. 11 657-660
[4]  
Fu W.(1999)Telomerase enzyme activity and RNA expression in adriamycin-resistant human breast carcinoma MCF-7 cells Cancer Let. 141 187-194
[5]  
Begley J. G.(1994)Specific association of human telomerase activity with immortal cells and cancer Science 266 2011-2015
[6]  
Killen M. W.(1995)Modifications of a telomeric repeat amplification protocol (TRAP) result in increased reliability, linearity and sensitivity Nucleic. Acids Res. 23 3794-3795
[7]  
Mattson M. P.(1997)Advances in qualification and characterization of telomerase activity by the telomeric repeat amplification protocol (TRAP) Nucleic. Acids Res. 25 2595-2597
[8]  
Nugent C. I.(1998)New method to measure telomerase activity by transcription-mediated amplification and hybridization protection assay Clin. Chem. 44 2446-2452
[9]  
Bosco G.(1998)Simplified method for the telomeric repeat amplification protocol (TRAP) Biotech. 24 726-728
[10]  
Ross L. O.(1998)Rapid, quantitative nonisotopic assay for telomerase activity in human tumors Clin. Chem. 44 2133-2138
12345678