Self-repair protects microtubules from destruction by molecular motors

被引：0

作者：

Sarah Triclin

Daisuke Inoue

Jérémie Gaillard

Zaw Min Htet

Morgan E. DeSantis

Didier Portran

Emmanuel Derivery

Charlotte Aumeier

Laura Schaedel

Karin John

Christophe Leterrier

Samara L. Reck-Peterson

Laurent Blanchoin

Manuel Théry

机构：

[1] University of Grenoble-Alpes,Interdisciplinary Research Institute of Grenoble, Laboratoire de Physiologie Cellulaire & Végétale, CytoMorpho Lab

[2] CEA,Department of Human Science, Faculty of Design

[3] CNRS,Deptartment of Cellular and Molecular Medicine, and Cell and Developmental Biology Section, Division of Biological Sciences

[4] INRA,CRBM

[5] Kyushu University,MRC Laboratory of Molecular Biology

[6] University of California San Diego,Department of Biochemistry

[7] University of Montpellier,Laboratoire Interdisciplinaire de Physique

[8] CNRS,Institut de Recherche Saint Louis, U976 Human Immunology Pathophysiology Immunotherapy (HIPI), CytoMorpho Lab

[9] Cambridge Biomedical Campus,undefined

[10] University of Geneva,undefined

[11] University of Grenoble-Alpes,undefined

[12] CNRS,undefined

[13] Aix-Marseille University,undefined

[14] CNRS,undefined

[15] Howard Hughes Medical Institute,undefined

[16] University of Paris,undefined

[17] INSERM,undefined

[18] CEA,undefined

来源：

Nature Materials | 2021年 / 20卷

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摘要：

Microtubule instability stems from the low energy of tubulin dimer interactions, which sets the growing polymer close to its disassembly conditions. Molecular motors use ATP hydrolysis to produce mechanical work and move on microtubules. This raises the possibility that the mechanical work produced by walking motors can break dimer interactions and trigger microtubule disassembly. We tested this hypothesis by studying the interplay between microtubules and moving molecular motors in vitro. Our results show that molecular motors can remove tubulin dimers from the lattice and rapidly destroy microtubules. We also found that dimer removal by motors was compensated for by the insertion of free tubulin dimers into the microtubule lattice. This self-repair mechanism allows microtubules to survive the damage induced by molecular motors as they move along their tracks. Our study reveals the existence of coupling between the motion of molecular motors and the renewal of the microtubule lattice.

引用

页码：883 / 891

页数：8

共 69 条

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