Variations of vinblastine accumulation and redox state affected by exogenous H2O2 in Catharanthus roseus (L.) G. Don

被引：0
作者：
Zhonghua Tang
Lei Yang
Yuangang Zu
Xiaorui Guo
机构：
[1] Ministry of Education,Key Laboratory of Forest Plant Ecology
[2] Northeast Forestry University,undefined
来源：
Plant Growth Regulation | 2009年 / 57卷
关键词：
(L.) G. Don; H; O; Redox state; Vinblastine;
D O I：
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学科分类号：
摘要：
Effects of exogenous H2O2 application on vinblastine (VBL) and its precursors, vindoline (VIN), catharanthine (CAT) and α-3′,4′-anhydrovinblastine (AVBL), were measured in Catharanthus roseus seedlings in order to explore possible correlation of VBL formation with oxidative stress. VBL accumulation has previously been shown to be regulated by an in vitro H2O2-dependent peroxidase (POD)-like synthase. Experimental exposure of plants to different concentrations of H2O2 showed that endogenous H2O2 and alkaloid concentrations in leaves were positively elevated. The time-course variations of alkaloid concentrations and redox state, reflected by the concentrations of H2O2, ascorbic acid (AA), oxidative product of glutathione (GSSG) and POD activity, were significantly altered due to H2O2 application. The further correlation analysis between alkaloids and redox status indicated that VBL production was tightly correlated with redox status. These results provide a new link between VBL metabolisms and redox state in C. roseus.
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页码：15 / 20
页数：5
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共 65 条
[1]  
Aert RJ(1996)Signaling molecules and the synthesis of alkaloids in Phytochemistry 42 417-422
[2]  
Schäfer A(1997) seedlings Plant Cell 9 1559-1572
[3]  
Hesse M(2004)Two distinct sources of elicited reactive oxygen species in Tobacco epidermal cells Plant Cell 16 1143-1162
[4]  
Baumann TW(1998)The ascorbic acid redox state controls guard cell signaling and stomatal movement Plant J 15 289-300
[5]  
Slusarenko A(2000)Enzymatic oxidations in the biosynthesis of complex alkaloids Trends Plant Sci 5 1360-1385
[6]  
Allan AC(1986)The cell and developmental biology of alkaloid biosynthesis J Plant Physiol 125 147-156
[7]  
Fluhr R(2001)Biosynthesis of indole alkaloids: developmental regulation of the biosynthetic pathway from tabersonine to vindoline in Annu Rev Plant Biol 52 29-66
[8]  
Chen Z(1991)Alkaloid biosynthesis in plants: biochemistry, cell biology, molecular regulation, and metabolic engineering applications Phytochemistry 30 3864-3874
[9]  
Gallie DR(2007)Bioactivity in plants: the link between phytochemistry and medicine FEBS J 274 1290-1303
[10]  
Chou WM(2005)Cloning, characterization and localization of a novel basic peroxidase gene from Chin J Anal Chem 33 89-92
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