NEDD8 nucleates a multivalent cullin–RING–UBE2D ubiquitin ligation assembly

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作者
Kheewoong Baek
David T. Krist
J. Rajan Prabu
Spencer Hill
Maren Klügel
Lisa-Marie Neumaier
Susanne von Gronau
Gary Kleiger
Brenda A. Schulman
机构
[1] Max Planck Institute of Biochemistry,Department of Molecular Machines and Signaling
[2] University of Nevada,Department of Chemistry and Biochemistry
[3] Las Vegas,Carl R. Woese Institute for Genomic Biology
[4] University of Illinois at Urbana-Champaign,undefined
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Nature | 2020年 / 578卷
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摘要
Eukaryotic cell biology depends on cullin–RING E3 ligase (CRL)-catalysed protein ubiquitylation1, which is tightly controlled by the modification of cullin with the ubiquitin-like protein NEDD82–6. However, how CRLs catalyse ubiquitylation, and the basis of NEDD8 activation, remain unknown. Here we report the cryo-electron microscopy structure of a chemically trapped complex that represents the ubiquitylation intermediate, in which the neddylated CRL1β-TRCP promotes the transfer of ubiquitin from the E2 ubiquitin-conjugating enzyme UBE2D to its recruited substrate, phosphorylated IκBα. NEDD8 acts as a nexus that binds disparate cullin elements and the RING-activated ubiquitin-linked UBE2D. Local structural remodelling of NEDD8 and large-scale movements of CRL domains converge to juxtapose the substrate and the ubiquitylation active site. These findings explain how a distinctive ubiquitin-like protein alters the functions of its targets, and show how numerous NEDD8-dependent interprotein interactions and conformational changes synergistically configure a catalytic CRL architecture that is both robust, to enable rapid ubiquitylation of the substrate, and fragile, to enable the subsequent functions of cullin–RING proteins.
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页码:461 / 466
页数:5
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