Immunofluorescent analysis of meiotic recombination in the domestic cat

被引：0
作者：
Borodin P.M. [1 ,2 ]
Karamysheva T.V. [1 ]
Rubtsov N.B. [1 ,2 ]
机构：
[1] Novosibirsk State University, Novosibirsk
来源：
Cell and Tissue Biology | 2007年 / 1卷 / 6期
基金：
俄罗斯基础研究基金会;
关键词：
Crossing over; Interference; Meiosis; MLH1; protein; Recombination; Synaptonemal complex;
D O I：
10.1134/S1990519X07060065
中图分类号：
学科分类号：
摘要：
The paper presents the analysis of the frequency, density, and distribution of recombination sites in the male meiosis of the domestic cat (Felis silvestris catus). The study was carried out using immunofluorescent staining of synaptonemal complex (SC) proteins, centromeric proteins and mismatch repair protein MLH1, a reliable marker of crossingover sites. We mapped 2633 sites of crossing over in 1098 individual autosomes. Based on these data, we estimated the total length of the genetic map of the domestic cat to be 2176 centimorgans. Positive correlation between the length of SC and the number of recombination sites common for mammalians was also found in the domestic cat. It was shown that this species was characterized by the highest density of recombination and the lowest interference in mammals. © Pleiades Publishing, Ltd. 2007.
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页码：503 / 507
页数：4
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