Immobilization of glucose oxidase on Fe3O4/SiO2 magnetic nanoparticles

被引：0
作者：
Jun Huang
Rong Zhao
Hai Wang
Wenqi Zhao
Liyun Ding
机构：
[1] Wuhan University of Technology,Key Laboratory of Fiber Optic Sensing Technology and Information Processing of Ministry of Education
来源：
Biotechnology Letters | 2010年 / 32卷
关键词：
Activity; Fe; O; /SiO; magnetic nanoparticles; Glucose oxidase; Immobilization; Stability;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Glucose oxidase (GOD) was covalently immobilized onto Fe3O4/SiO2 magnetic nanoparticles (FSMNs) using glutaraldehyde (GA). Optimal immobilization was at pH 6 with 3-aminopropyltriethoxysilane at 2% (v/v), GA at 3% (v/v) and 0.143 g GOD per g carrier. The activity of immobilized GOD was 4,570 U/g at pH 7 and 50°C. The immobilized GOD retained 80% of its initial activity after 6 h at 45°C while free enzyme retained only 20% activity. The immobilized GOD maintained 60% of its initial activity after 6 cycles of repeated use and retained 75% of its initial activity after 1 month at 4°C whereas free enzymes retained 62% of its activity.
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页码：817 / 821
页数：4
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